Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis.

Détails

ID Serval
serval:BIB_077830B5EB4C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis.
Périodique
Molecular Endocrinology
Auteur⸱e⸱s
Zimmermann C., Stévant I., Borel C., Conne B., Pitetti J.L., Calvel P., Kaessmann H., Jégou B., Chalmel F., Nef S.
ISSN
1944-9917 (Electronic)
ISSN-L
0888-8809
Statut éditorial
Publié
Date de publication
2015
Volume
29
Numéro
4
Pages
627-642
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Sertoli cells (SCs), the only somatic cells within seminiferous tubules, associate intimately with developing germ cells. They not only provide physical and nutritional support but also secrete factors essential to the complex developmental processes of germ cell proliferation and differentiation. The SC transcriptome must therefore adapt rapidly during the different stages of spermatogenesis. We report comprehensive genome-wide expression profiles of pure populations of SCs isolated at 5 distinct stages of the first wave of mouse spermatogenesis, using RNA sequencing technology. We were able to reconstruct about 13 901 high-confidence, nonredundant coding and noncoding transcripts, characterized by complex alternative splicing patterns with more than 45% comprising novel isoforms of known genes. Interestingly, roughly one-fifth (2939) of these genes exhibited a dynamic expression profile reflecting the evolving role of SCs during the progression of spermatogenesis, with stage-specific expression of genes involved in biological processes such as cell cycle regulation, metabolism and energy production, retinoic acid synthesis, and blood-testis barrier biogenesis. Finally, regulatory network analysis identified the transcription factors endothelial PAS domain-containing protein 1 (EPAS1/Hif2α), aryl hydrocarbon receptor nuclear translocator (ARNT/Hif1β), and signal transducer and activator of transcription 1 (STAT1) as potential master regulators driving the SC transcriptional program. Our results highlight the plastic transcriptional landscape of SCs during the progression of spermatogenesis and provide valuable resources to better understand SC function and spermatogenesis and its related disorders, such as male infertility.
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/05/2015 9:03
Dernière modification de la notice
20/08/2019 12:29
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