Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis.

Détails

ID Serval
serval:BIB_06B33D0B7207
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization and recruitment of plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis.
Périodique
Journal of Immunology
Auteur(s)
Lande R., Giacomini E., Serafini B., Rosicarelli B., Sebastiani G.D., Minisola G., Tarantino U., Riccieri V., Valesini G., Coccia E.M.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
2004
Volume
173
Numéro
4
Pages
2815-2824
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Dendritic cells (DCs) are thought to play a key role in driving the immunopathogenic response underlying chronic inflammatory arthritis. In this study, we have examined the presence and phenotype of plasmacytoid DCs (pDCs) in the synovial fluids (SF) of patients with rheumatoid arthritis (RA), psoriatic arthritis (PA), and osteoarthritis (OA) and determined the chemotactic properties of SF from these patients toward pDCs. Flow cytometry analysis showed that the percentage of pDCs, identified as a population of Lin(-)CD123(++) cells, is 4- to 5-fold higher in RA SF and PA SF than in OA SF. The morphological and immunophenotypic characterization of pDCs isolated from PA and RA SF indicates that they are in an immature state, most likely due to inhibitory factors present in RA SF, but are still able to undergo maturation when exposed ex vivo to viral agent or unmethylated DNA. CD123(+) and BDCA2(+) pDCs were detected by immunohistochemistry in RA synovial tissue in which expression of the IFN-alpha-inducible protein MxA was also found, suggesting production of type I IFN by maturing pDCs. We also show that CXCR3 and CXCR4 are expressed by both blood-derived pDCs and pDCs isolated from RA and PA SF and that CXCL-10, CXCL-11, and CXCL-12 present in RA and PA SF stimulate chemotaxis of blood-derived pDCs. Altogether, these findings suggest that chemokine-driven recruitment of pDCs from the blood to the inflamed synovium could be important in the regulation of the immune response in chronic inflammatory arthritis.
Mots-clé
Antigens, Surface/immunology, Arthritis/immunology, Arthritis/physiopathology, Cell Differentiation/immunology, Chemokine CXCL10, Chemokine CXCL11, Chemokine CXCL12, Chemokines, CXC/metabolism, Chemotaxis, Leukocyte/immunology, Chronic Disease, Dendritic Cells/cytology, Dendritic Cells/physiology, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Immunohistochemistry, Immunophenotyping, Interferon Type I/biosynthesis, Interleukin-3 Receptor alpha Subunit, Phenotype, Receptors, CXCR3, Receptors, CXCR4/biosynthesis, Receptors, Chemokine/biosynthesis, Receptors, Interleukin-3/metabolism, Synovial Fluid/cytology, Synovial Fluid/immunology, Synovial Membrane/cytology, Synovial Membrane/immunology
Pubmed
Web of science
Création de la notice
04/02/2013 12:10
Dernière modification de la notice
20/08/2019 12:28
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