Viral vectors for opto-electrode recording and photometry-based imaging of oxytocin neurons in anesthetized and socially interacting rats.
Détails
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_067D68F599E8
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Viral vectors for opto-electrode recording and photometry-based imaging of oxytocin neurons in anesthetized and socially interacting rats.
Périodique
STAR protocols
ISSN
2666-1667 (Electronic)
ISSN-L
2666-1667
Statut éditorial
Publié
Date de publication
18/03/2022
Peer-reviewed
Oui
Volume
3
Numéro
1
Pages
101032
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
Here, we present a step-by-step protocol to target, record, and manipulate the activity of oxytocin neurons in awake rats. The protocol includes a procedure to record the activity of oxytocin neurons from awake and socially interacting rats using opto-electrodes for simultaneous electrophysiological recording and virally based cell-type-specific opto-tagging with Channelrhodopsin 2. Furthermore, we illustrate a procedure for optically guided implantation of optic fiber and imaging of oxytocin neuron population activity expressing calcium indicator GCaMP6s with the fiber photometry technique. For complete details on the use and execution of this protocol, please refer to Tang et al., 2020.
Mots-clé
Animals, Electrodes, Fiber Optic Technology/methods, Neurons/physiology, Oxytocin, Photometry/methods, Rats, Behavior, Biotechnology and bioengineering, Microscopy, Model Organisms, Neuroscience
Pubmed
Open Access
Oui
Création de la notice
11/01/2022 14:52
Dernière modification de la notice
25/01/2024 8:31