The purpose of this study was to compare the affects of drafting or a reduction of exercise intensity during swimming on the power output sustained (P(mean)) during a subsequent cycle time trial (TT). In addition the relationship between peak power output (PPO) and P(mean) generated during the cycle TT after swimming was examined. Nine well-trained triathletes performed an incremental cycling test to exhaustion for determination of PPO. In addition, each subject performed three swim-cycle (SC) trials consisting of 20 min cycle TT preceded by a 400 m swimming trial completed as (1) "all out" and in a non-drafting situation (SC(100%)); (2) at 90% of SC(100%) in a non-drafting situation (SC(90%)); (3) in a drafting position at the same controlled velocity as SC(100%) (SC(drafting)). Swimming velocity (ms(-1)) was significantly (p<0.01) lower at each time point during the 400 m swimming trial in SC(90%) compared with SC(100%) and SC(drafting). There was no significant difference in velocity between SC(100%) and SC(drafting). Blood lactate (BLA) concentration was also significantly (p<0.01) lower after swimming in SC(90%) compared to SC(100%) and SC(drafting) (3.8+/-0.9 versus 7.3+/-2.4 and 7.9+/-2.4mM). The Pmean was also significantly (p<0.05) lower in SC(100%) relative to the SC(90%) and SC(drafting) (226+/-15 versus 253+/-33 and 249+/-36W). There was no significant correlation between PPO (W) and P(mean) for SC(100%) (r=-0.32), SC(90%) (r=0.65; p=0.058) or SC(drafting) (r=0.54). This study indicates that drafting or swimming at a lower velocity did not induce any conflicting affects on power output during a subsequent cycle TT. However, this study confirms that P(mean) during a cycle TT is reduced when prior swimming is performed. Furthermore the positive relationship typically observed between PPO and P(mean) is disrupted by swimming activity performed before a cycling TT. This factor should be considered in terms of physiological analysis of triathletes.