Systems biology of macrophages.

Détails

ID Serval
serval:BIB_066E708B23B3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Systems biology of macrophages.
Périodique
Advances in experimental medicine and biology
Auteur⸱e⸱s
Maurya M.R., Benner C., Pradervand S., Glass C., Subramaniam S.
ISSN
0065-2598 (Print)
ISSN-L
0065-2598
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
598
Pages
62-79
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
Publication Status: ppublish
Résumé
Cells and tissues function in context. Under a given growth or survival medium they perform tasks, replicate and die. Given a stimulus they respond by invoking myriad biomolecular networks that result in a specified cellular outcome. At any given instant it can be argued that the cell is in a "state" defined by its components--their concentrations and locations, the interactions between components--that are modulated in space and time, and the complex circuitry--that involves a large number of interacting networks and a snapshot of the dynamical processes--such as gene expression, cell cycle, transport of components, etc. At present, we can measure, using high and low throughput methods, several cellular components in a context-dependent manner and obtain a partial picture of cellular networks and dynamical processes. Are these measurements sufficient to answer important biological questions and help reconstruct a systems-level understanding of a mammalian cell? This chapter will address systems biology strategies developed to address this question and demonstrate the power of integration of diverse cellular data for answering interesting biological questions in macrophages. We will use this systems biology approach to address the following questions: (1) How good are macrophage cell lines in addressing phenotypic biology of primary macrophages? (2) How do signals associated with inflammatory molecules regulate gene transcription in macrophages? (3) How can we combine proteomic and other cellular measurements to characterize the repertoire of upstream signaling networks invoked by macrophages? (4) How do designed knockdowns of proteins influence cellular phenotypes?
Mots-clé
Animals, Calcium/metabolism, Cell Line, Complement C5a/immunology, GTP Phosphohydrolases/metabolism, Gene Expression Profiling, Inositol 1,4,5-Trisphosphate/metabolism, Lipopolysaccharides/immunology, Macrophages/cytology, Macrophages/physiology, Mathematics, Models, Theoretical, Oligonucleotide Array Sequence Analysis, Signal Transduction/physiology
Pubmed
Web of science
Création de la notice
18/12/2014 16:34
Dernière modification de la notice
22/03/2024 16:42
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