Electrospray micromixer chip for on-line derivatization and kinetic studies.

Détails

ID Serval
serval:BIB_061E74CFEF88
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electrospray micromixer chip for on-line derivatization and kinetic studies.
Périodique
Analytical Chemistry
Auteur(s)
Abonnenc M., Dayon L., Perruche B., Lion N., Girault H.H.
ISSN
1520-6882 (Electronic)
ISSN-L
0003-2700
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
80
Numéro
9
Pages
3372-3378
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid junction. Downstream of this liquid junction, a mixing unit made of parallel oblique grooves is integrated to the microchannel in order to create flow perturbations. Several mixer designs are evaluated. The mixer efficiency is investigated both by fluorescence study and mass spectrometric monitoring of the tagging reaction of cysteinyl peptides with 1,4-benzoquinone. The comparisons with a microchip without a mixing unit and a kinetic model are used to assess the efficiency of the mixer showing tagging kinetics close to that of bulk reactions in an ideally mixed reactor. As an ultimate application, the electrospray micromixer is implemented in a LC-MS workflow. On-line derivatization of albumin tryptic peptides after a reversed-phase separation and counting of their cysteines drastically enhance the protein identification.
Mots-clé
Benzoquinones/chemistry, Chromatography, High Pressure Liquid/methods, Cysteine/chemistry, Image Processing, Computer-Assisted, Kinetics, Microchip Analytical Procedures/instrumentation, Microchip Analytical Procedures/methods, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Models, Chemical, Peptide Fragments/chemistry, Peptides/analysis, Peptides/chemistry, Photochemistry, Serum Albumin, Bovine/chemistry, Spectrometry, Mass, Electrospray Ionization/instrumentation, Spectrometry, Mass, Electrospray Ionization/methods, Trypsin/chemistry, Ultraviolet Rays
Pubmed
Web of science
Création de la notice
09/11/2014 22:44
Dernière modification de la notice
20/08/2019 12:28
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