Assessment of different sources of variation in the antibody responses to specific malaria antigens in children in Papua New Guinea

Détails

ID Serval
serval:BIB_05D7515DDE5E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Assessment of different sources of variation in the antibody responses to specific malaria antigens in children in Papua New Guinea
Périodique
International Journal of Epidemiology
Auteur(s)
Stirnadel  H. A., Al-Yaman  F., Genton  B., Alpers  M. P., Smith  T. A.
ISSN
0300-5771 (Print)
Statut éditorial
Publié
Date de publication
06/2000
Volume
29
Numéro
3
Pages
579-86
Notes
Journal Article --- Old month value: Jun
Résumé
BACKGROUND: A potential problem for malaria vaccine development and testing is between-host variation in antibody responses to specific malaria antigens. Previous work in adults in an area highly endemic for Plasmodium falciparum in Papua New Guinea found that genetic regulation partly explained heterogeneity in responsiveness. We have now assessed the relative contributions of environmental and genetic factors in total IgG responses to specific malaria antigens in children, and quantified temporal variation within individuals of total IgG responses. METHODS: Total IgG responses against schizont extract, merozoite surface protein-1, merozoite surface protein-2, ring-infected erythrocyte surface antigen, and SPf66 were measured by ELISA. Variance component analysis was used to estimate the variation explained by genetic and environmental factors in these antibody responses. Intra- and inter-class correlations of antibody responses within relative pairs were estimated. We adjusted for age, P. falciparum density, sex and village differences either within or prior to the analysis. RESULTS: For all malaria antigens, temporal variation in the total IgG response was the predominant source of variation. There was substantial familial aggregation of all IgG responses, but it remained unclear how much this clustering was attributable to genetic factors and how much to a common environment in the household. The remaining variance, which could not be explained by either of the above, was very small for most of the antigens. CONCLUSIONS: Temporal variation and clustering of immune responses to specific malaria antigens need to be taken into account when planning, conducting and interpreting immuno-epidemiological and vaccine studies.
Mots-clé
Animals Antibody Formation Antigens, Protozoan/immunology Child Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G/analysis/genetics/*immunology Malaria/*immunology Malaria Vaccines/*immunology Male Pedigree Plasmodium falciparum/immunology Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:48
Dernière modification de la notice
20/08/2019 12:27
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