Absorbance enhancement in microplate wells for improved-sensitivity biosensors

Détails

Ressource 1Télécharger: BIB_05D734F911CD.P001.pdf (507.68 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_05D734F911CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Absorbance enhancement in microplate wells for improved-sensitivity biosensors
Périodique
Biosensors and Bioelectronics
Auteur(s)
Suárez Guillaume, Santschi Christian, Plateel Gregory, Martin Olivier J., Riediker Michael
ISSN
1873-4235 (Electronic)
ISSN-L
0956-5663
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
56
Pages
198-203
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
A generic optical biosensing strategy was developed that relies on the absorbance enhancement phenomenon occurring in a multiple scattering matrix. Experimentally, inserts made of glass fiber membrane were placed into microplate wells in order to significantly lengthen the trajectory of the incident light through the sample and therefore increase the corresponding absorbance. Enhancement factor was calculated by comparing the absorbance values measured for a given amount of dye with and without the absorbance-enhancing inserts in the wells. Moreover, the dilution of dye in solutions with different refractive indices (RI) clearly revealed that the enhancement factor increased with the ΔRI between the membrane and the surrounding medium, reaching a maximum value (EF>25) when the membranes were dried. On this basis, two H2O2-biosensing systems were developed based on the biofunctionalization of the glass fiber inserts either with cytochrome c or horseradish peroxidase (HRP) and the analytical performances were systematically compared with the corresponding bioassay in solution. The efficiency of the absorbance-enhancement approach was particularly clear in the case of the cytochrome c-based biosensor with a sensitivity gain of 40 folds and wider dynamic range. Therefore, the developed strategy represents a promising way to convert standard colorimetric bioassays into optical biosensors with improved sensitivity.
Mots-clé
Biosensing Techniques/methods , Cytochromes c/chemistry , Cytochromes c/metabolism , Enzymes, Immobilized/chemistry , Enzymes, Immobilized/metabolism , Glass/chemistry , Horseradish Peroxidase/chemistry , Horseradish Peroxidase/metabolism , Hydrogen Peroxide/analysis , Hydrogen Peroxide/metabolism , Models, Molecular , Refractometry ,
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/02/2014 17:38
Dernière modification de la notice
20/08/2019 12:27
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