CRISPR System Acquisition and Evolution of an Obligate Intracellular Chlamydia-Related Bacterium.

Détails

Ressource 1Télécharger: BIB_044EE19F2770.P001.pdf (2629.57 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_044EE19F2770
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CRISPR System Acquisition and Evolution of an Obligate Intracellular Chlamydia-Related Bacterium.
Périodique
Genome Biology and Evolution
Auteur(s)
Bertelli C., Cissé O.H., Rusconi B., Kebbi-Beghdadi C., Croxatto A., Goesmann A., Collyn F., Greub G.
ISSN
1759-6653 (Electronic)
ISSN-L
1759-6653
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
8
Numéro
8
Pages
2376-2386
Langue
anglais
Résumé
Recently, a new Chlamydia-related organism, Protochlamydia naegleriophila KNic, was discovered within a Naegleria amoeba. To decipher the mechanisms at play in the modeling of genomes from the Protochlamydia genus, we sequenced the full genome of Pr. naegleriophila, which includes a 2,885,090 bp chromosome and a 145,285 bp megaplasmid. For the first time within the Chlamydiales order, we describe the presence of a clustered regularly interspaced short palindromic repeats (CRISPR) system, the immune system of bacteria, located on the chromosome. It is composed of a small CRISPR locus comprising eight repeats and associated cas-cse genes of the subtype I-E. A CRISPR locus is also present within Chlamydia sp. Diamant, another Pr. naegleriophila strain, suggesting that the CRISPR system was acquired by a common ancestor of Pr. naegleriophila, after its divergence from Pr. amoebophila. Both nucleotide bias and comparative genomics approaches identified probable horizontal gene acquisitions within two and four genomic islands in Pr. naegleriophila KNic and Diamant genomes, respectively. The plasmid encodes an F-type conjugative system highly similar to 1) that found in the Pam100G genomic island of Pr. amoebophila UWE25 chromosome, as well as on the plasmid of Rubidus massiliensis and 2) to the three genes remaining in the chromosome of Parachlamydia acanthamoebae strains. Therefore, this conjugative system was likely acquired on an ancestral plasmid before the divergence of Parachlamydiaceae Overall, this new complete Pr. naegleriophila genome sequence enables further investigation of the dynamic processes shaping the genomes of the family Parachlamydiaceae and the genus Protochlamydia.
Pubmed
Open Access
Oui
Création de la notice
15/09/2016 21:28
Dernière modification de la notice
20/08/2019 13:26
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