DNA transformations of Candida tropicalis with replicating and integrative vectors
Détails
ID Serval
serval:BIB_04061F637F39
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DNA transformations of Candida tropicalis with replicating and integrative vectors
Périodique
Yeast
ISSN
0749-503X (Print)
Statut éditorial
Publié
Date de publication
12/1992
Volume
8
Numéro
12
Pages
1065-75
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
The alkane-assimilating yeast Candida tropicalis was used as a host for DNA transformations. A stable ade2 mutant (Ha900) obtained by UV-mutagenesis was used as a recipient for different vectors carrying selectable markers. A first vector, pMK16, that was developed for the transformation of C. albicans and carries an ADE2 gene marker and a Candida autonomously replicating sequence (CARS) element promoting autonomous replication, was compatible for transforming Ha900. Two transformant types were observed: (i) pink transformants which easily lose pMK16 under non-selective growth conditions; (ii) white transformants, in which the same plasmid exhibited a higher mitotic stability. In both cases pMK16 could be rescued from these cells in Escherichia coli. A second vector, pADE2, containing the isolated C. tropicalis ADE2, gene, was used to transform Ha900. This vector integrated in the yeast genome at homologous sites of the ade2 locus. Different integration types were observed at one or both ade2 alleles in single or in tandem repeats.
Mots-clé
Alcohol Dehydrogenase/genetics
Candida/*genetics
Candida albicans/genetics
Cell Fusion
Cloning, Molecular
DNA Replication
DNA, Fungal/*genetics
Escherichia coli/genetics
Genetic Complementation Test
Genetic Markers/genetics
Genetic Vectors/*genetics
Genome, Fungal
Plasmids/*genetics
Protoplasts
Repetitive Sequences, Nucleic Acid/genetics
Restriction Mapping
*Transformation, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 15:40
Dernière modification de la notice
20/08/2019 13:25