Immunocytochemical expression of monocarboxylate transporters in the human visual cortex at midgestation

Détails

ID Serval
serval:BIB_039F7F2DAD08
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunocytochemical expression of monocarboxylate transporters in the human visual cortex at midgestation
Périodique
Brain Research. Developmental Brain Research
Auteur(s)
Fayol  L., Baud  O., Monier  A., Pellerin  L., Magistretti  P., Evrard  P., Verney  C.
ISSN
0165-3806 (Print)
Statut éditorial
Publié
Date de publication
01/2004
Volume
148
Numéro
1
Pages
69-76
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 31
Résumé
Lactate and the other monocarboxylates are a major energy source for the developing brain. We investigated the immunocytochemical expression of two monocarboxylate transporters, MCT1 and MCT2, in the human visual cortex between 13 and 26 post-ovulatory weeks. We used immunoperoxidase and immunofluorescence techniques to determine whether these transporters co-localized with markers for blood vessels (CD34), neurons (microtubule-associated protein 2 [MAP2], SMI 311), radial glia (vimentin), or astrocytes (glial fibrillary acidic protein [GFAP], S100beta protein). MCT1 immunoreactivity was visible in blood vessel walls as early as the 13th week of gestation mainly in the cortical plate and subplate. At this stage, less than 10% of vessels in the ventricular layer expressed MCT1, whereas all blood vessels walls showed this immunoreactivity at the 26th gestational week. Starting at the 19th week of gestation, sparse MCT1 positive cell bodies were detected, some of them co-localized with MAP2 immunoreactivity. MCT2 immunoreactivity was noted in astrocytic cell bodies from week 19 and spread subsequently to the astrocyte end-feet in contact with blood vessels. MCTs immunoreactivities were most marked in the subplate and deep cortical plate, where the most differentiated neurons were located. Our findings suggest that monocarboxylate trafficking between vessels (MCT1), astrocytes (MCT2) and some postmitotic neurons (MCT1) could develop gradually toward 20 gestational weeks (g.w.). These data suggest that lactate or other monocarboxylates could represent a significant energy source for the human visual cortex at this early stage.
Mots-clé
Antigens, CD34/metabolism Blood Vessels/embryology/metabolism Cell Cycle Proteins/*metabolism Fetus *Gestational Age Glial Fibrillary Acidic Protein/metabolism Glucose Transport Proteins, Facilitative Humans Immunohistochemistry/methods Microtubule-Associated Proteins/metabolism Monocarboxylic Acid Transporters/*metabolism Monosaccharide Transport Proteins/metabolism Nerve Growth Factors/metabolism Oncogene Proteins/*metabolism S100 Proteins/metabolism Vimentin/metabolism Visual Cortex/cytology/embryology/*metabolism
Pubmed
Création de la notice
24/01/2008 13:16
Dernière modification de la notice
20/08/2019 12:25
Données d'usage