Upregulation of IRAS/nischarin (I1-imidazoline receptor), a regulatory protein of μ-opioid receptor trafficking, in postmortem prefrontal cortex of long-term opiate and mixed opiate/cocaine abusers.

Détails

ID Serval
serval:BIB_038F6B364D05
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Upregulation of IRAS/nischarin (I1-imidazoline receptor), a regulatory protein of μ-opioid receptor trafficking, in postmortem prefrontal cortex of long-term opiate and mixed opiate/cocaine abusers.
Périodique
Neurochemistry international
Auteur(s)
Keller B., La Harpe R., García-Sevilla J.A.
ISSN
1872-9754 (Electronic)
ISSN-L
0197-0186
Statut éditorial
Publié
Date de publication
09/2017
Peer-reviewed
Oui
Volume
108
Pages
282-286
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
PDF: Rapid communication
Résumé
Imidazoline receptor antisera-selected (IRAS)/nischarin, a putative I1-imidazoline receptor, has recently been shown to regulate μ-opioid receptor (OR) trafficking and resensitisation. To study a possible involvement of this μ-OR regulator in opiate dependence, the present study assessed by Western blot analysis the contents of IRAS/nischarin and μ-OR in total homogenates and subcellular preparations of postmortem human prefrontal cortex (PFC/BA9) of long-term opiate and mixed opiate/cocaine abusers as well as of matched healthy control subjects. In the PFC/BA9 of long-term opiate/cocaine abusers (all subjects together) IRAS/nischarin content was increased (+67%, p < 0.01, n = 11) when compared with matched controls (n = 10). Similar increases were found for the subgroups of opiate (+72%, n = 6) and mixed opiate/cocaine (+61%, n = 5) abusers. IRAS/nischarin immunocontents were also found increased in subcellular membrane preparations (+61%, p < 0.05, n = 10) of PFC/BA9 from opiate addicts. In the same brain samples, the levels of μ-OR were not different to those in control subjects. Based on the increased contents in brains of opiate abusers and the reported function as μ-OR regulator, IRAS/nischarin could represent a new promising target for treatment of opiate use disorder.

Mots-clé
IRAS/nischarin, Imidazoline receptors, Opiate dependence, μ-Opioid receptor
Pubmed
Web of science
Création de la notice
04/05/2017 13:59
Dernière modification de la notice
20/08/2019 12:25
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