Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor.

Détails

ID Serval
serval:BIB_03777D375057
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Two-Dimensional Label-Free Affinity Analysis of Tumor-Specific CD8 T Cells with a Biomimetic Plasmonic Sensor.
Périodique
ACS sensors
Auteur(s)
Soler M., Li X., John-Herpin A., Schmidt J., Coukos G., Altug H.
ISSN
2379-3694 (Electronic)
ISSN-L
2379-3694
Statut éditorial
Publié
Date de publication
26/11/2018
Peer-reviewed
Oui
Volume
3
Numéro
11
Pages
2286-2295
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The screening and analysis of T cells functional avidity for specific tumor-associated antigens is crucial for the development of personalized immunotherapies against cancer. The affinity and kinetics of a T cell receptor (TCR) binding to the peptide-major histocompatibility complex (pMHC), expressed on tumor or antigen-presenting cells, have shown major implications in T cell activation and effector functions. We introduce an innovative methodology for the two-dimensional affinity analysis of TCR-pMHC in a label-free configuration by employing a multiparametric Surface Plasmon Resonance biosensor (MP-SPR) functionalized with artificial cell membranes. The biomimetic scaffold created with planar lipid bilayers is able to efficiently capture the specific and intact tumor-specific T cells and monitor the formation of the immunological synapse in situ. We have achieved excellent limits of detection for in-flow cell capturing, up to 2 orders of magnitude below the current state-of-the-art for plasmonic sensing. We demonstrate the accuracy and selectivity of our sensor for the analysis of CD8 <sup>+</sup> T cells bioengineered with TCR of incremental affinities specific for the HLA-A0201/NY-ESO-I <sub>157-165</sub> pMHC complex. The study confirmed the significance of providing a biomimetic microenvironment, compared to the traditional molecular analysis, and showed fine agreement with previous results employing flow cytometry. Our methodology is reliable and versatile; thus, it can be applied to more sophisticated photonic and nanoplasmonic technologies for the screening of multiple cell types and boost the development of novel treatments for cancer.
Mots-clé
Biomimetic Materials/chemistry, Biosensing Techniques/methods, CD3 Complex/immunology, CD8-Positive T-Lymphocytes/immunology, Cell Line, Tumor, Cell Separation/methods, HLA-A2 Antigen/immunology, Humans, Limit of Detection, Lipid Bilayers/chemistry, Neoplasm Proteins/immunology, Neoplasms/immunology, Peptide Fragments/immunology, Phosphatidylcholines/chemistry, Phosphatidylethanolamines/chemistry, Phosphatidylserines/chemistry, Receptors, Antigen, T-Cell/immunology, Surface Plasmon Resonance/methods, TCR affinity analysis, artificial cell membrane, cell capture, immunotherapy, lipid bilayer, real-time analysis, surface plasmon resonance
Pubmed
Web of science
Création de la notice
29/10/2018 12:49
Dernière modification de la notice
03/10/2019 5:09
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