Processing of some antigens by the standard proteasome but not by the immunoproteasome results in poor presentation by dendritic cells

Détails

ID Serval
serval:BIB_036ED1B8451F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Processing of some antigens by the standard proteasome but not by the immunoproteasome results in poor presentation by dendritic cells
Périodique
Immunity
Auteur(s)
Morel  S., Levy  F., Burlet-Schiltz  O., Brasseur  F., Probst-Kepper  M., Peitrequin  A. L., Monsarrat  B., Van Velthoven  R., Cerottini  J. C., Boon  T., Gairin  J. E., Van den Eynde  B. J.
ISSN
1074-7613 (Print)
Statut éditorial
Publié
Date de publication
01/2000
Volume
12
Numéro
1
Pages
107-17
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
By stimulating human lymphocytes with an autologous renal carcinoma, we obtained CTL recognizing an antigen derived from a novel, ubiquitous protein. The CTL failed to lyse autologous EBV-transformed B cells, even though the latter express the protein. This is due to the presence in these cells of immunoproteasomes, which, unlike standard proteasomes, cannot produce the antigenic peptide. We show that dendritic cells also carry immunoproteasomes and fail to present this antigen. This may explain why the relevant CTL escape thymic deletion and are not regularly activated in the periphery. Lack of cleavage by the immunoproteasome was also observed for melanoma differentiation antigen Melan-A26-35/HLA-A2, currently used for antitumoral vaccination. For immunization with such antigens, proteins should be less suitable than peptides, which do not require proteasome digestion in dendritic cells.
Mots-clé
Antigen Presentation/*immunology Antigens, Neoplasm/genetics/*immunology B-Lymphocytes/immunology Base Sequence Cell Line, Transformed Cysteine Endopeptidases/genetics/*immunology DNA, Complementary Dendritic Cells/*immunology Herpesvirus 4, Human/immunology Humans Kidney Neoplasms/immunology Melanoma/immunology Molecular Sequence Data Multienzyme Complexes/*immunology Neoplasm Proteins/immunology Peptides/immunology Proteasome Endopeptidase Complex Proteins/genetics/immunology T-Lymphocytes, Cytotoxic/immunology Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 11:17
Dernière modification de la notice
20/08/2019 12:25
Données d'usage