Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.

Détails

ID Serval
serval:BIB_035E0D54C14B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory.
Périodique
Journal of Clinical Microbiology
Auteur(s)
Bizzini A., Durussel C., Bille J., Greub G., Prod'hom G.
ISSN
1098-660X[electronic], 0095-1137[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
48
Numéro
5
Pages
1549-1554
Langue
anglais
Résumé
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. In the present study, we prospectively compared MALDI-TOF MS to the conventional phenotypic method for the identification of routine isolates. Colonies were analyzed by MALDI-TOF MS either by direct deposition on the target plate or after a formic acid-acetonitrile extraction step if no valid result was initially obtained. Among 1,371 isolates identified by conventional methods, 1,278 (93.2%) were putatively identified to the species level by MALDI-TOF MS and 73 (5.3%) were identified to the genus level, but no reliable identification was obtained for 20 (1.5%). Among the 1,278 isolates identified to the species level by MALDI-TOF MS, 63 (4.9%) discordant results were initially identified. Most discordant results (42/63) were due to systematic database-related taxonomical differences, 14 were explained by poor discrimination of the MALDI-TOF MS spectra obtained, and 7 were due to errors in the initial conventional identification. An extraction step was required to obtain a valid MALDI-TOF MS identification for 25.6% of the 1,278 valid isolates. In conclusion, our results show that MALDI-TOF MS is a fast and reliable technique which has the potential to replace conventional phenotypic identification for most bacterial strains routinely isolated in clinical microbiology laboratories.
Mots-clé
Species Identification, Rapid Discrimination, Escherichia-Coli, Nov
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/05/2010 9:54
Dernière modification de la notice
20/08/2019 12:25
Données d'usage