Chromosome organization shapes replisome dynamics in Caulobacter crescentus.

Détails

ID Serval
serval:BIB_0328D0EC537F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Chromosome organization shapes replisome dynamics in Caulobacter crescentus.
Périodique
Nature communications
Auteur⸱e⸱s
Zhang C., Joseph A.M., Casini L., Collier J., Badrinarayanan A., Manley S.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
24/04/2024
Peer-reviewed
Oui
Volume
15
Numéro
1
Pages
3460
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
Publication Status: epublish
Résumé
DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial species, driving the hypothesis that a physical linker may tether them together. However, replisome splitting has also been reported in many of the same species, leaving the principles behind replisome organization a long-standing puzzle. Here, by tracking the replisome β-clamp subunit in live Caulobacter crescentus, we find that rapid DNA segregation can give rise to a second focus which resembles a replisome, but does not replicate DNA. Sister replisomes can remain colocalized, or split apart to travel along DNA separately upon disruption of chromosome inter-arm alignment. Furthermore, chromosome arm-specific replication-transcription conflicts differentially modify replication speed on the two arms, facilitate the decoupling of the two replisomes. With these observations, we conclude that the dynamic chromosome organization flexibly shapes the organization of sister replisomes, and we outline principles which can help to reconcile previously conflicting models of replisome architecture.
Mots-clé
Caulobacter crescentus/metabolism, Caulobacter crescentus/genetics, DNA Replication, Chromosomes, Bacterial/metabolism, Chromosomes, Bacterial/genetics, Bacterial Proteins/metabolism, Bacterial Proteins/genetics, DNA, Bacterial/metabolism, DNA, Bacterial/genetics, Chromosome Segregation
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/04/2024 13:37
Dernière modification de la notice
25/05/2024 7:12
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