High-resolution spatial mapping of changes in the neurochemical profile after focal ischemia in mice.

Détails

ID Serval
serval:BIB_030D9B1BCC50
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
High-resolution spatial mapping of changes in the neurochemical profile after focal ischemia in mice.
Périodique
NMR in Biomedicine
Auteur(s)
Alf M.F., Lei H., Berthet C., Hirt L., Gruetter R., Mlynarik V.
ISSN
1099-1492 (Electronic)
ISSN-L
0952-3480
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
25
Numéro
2
Pages
247-254
Langue
anglais
Notes
Publication types: Journal Article
Résumé
After ischemic stroke, the ischemic damage to brain tissue evolves over time and with an uneven spatial distribution. Early irreversible changes occur in the ischemic core, whereas, in the penumbra, which receives more collateral blood flow, the damage is more mild and delayed. A better characterization of the penumbra, irreversibly damaged and healthy tissues is needed to understand the mechanisms involved in tissue death. MRSI is a powerful tool for this task if the scan time can be decreased whilst maintaining high sensitivity. Therefore, we made improvements to a (1) H MRSI protocol to study middle cerebral artery occlusion in mice. The spatial distribution of changes in the neurochemical profile was investigated, with an effective spatial resolution of 1.4 μL, applying the protocol on a 14.1-T magnet. The acquired maps included the difficult-to-separate glutamate and glutamine resonances and, to our knowledge, the first mapping of metabolites γ-aminobutyric acid and glutathione in vivo, within a metabolite measurement time of 45 min. The maps were in excellent agreement with findings from single-voxel spectroscopy and offer spatial information at a scan time acceptable for most animal models. The metabolites measured differed with respect to the temporal evolution of their concentrations and the localization of these changes. Specifically, lactate and N-acetylaspartate concentration changes largely overlapped with the T(2) -hyperintense region visualized with MRI, whereas changes in cholines and glutathione affected the entire middle cerebral artery territory. Glutamine maps showed elevated levels in the ischemic striatum until 8 h after reperfusion, and until 24 h in cortical tissue, indicating differences in excitotoxic effects and secondary energy failure in these tissue types. Copyright © 2011 John Wiley & Sons, Ltd.
Pubmed
Web of science
Création de la notice
16/01/2012 13:47
Dernière modification de la notice
20/08/2019 12:25
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