Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells

Détails

ID Serval
serval:BIB_03043A3984BA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regulation of leukocyte adhesion molecule-1 (TQ1, Leu-8) expression and shedding by normal and malignant cells
Périodique
Leukemia
Auteur⸱e⸱s
Spertini  O., Freedman  A. S., Belvin  M. P., Penta  A. C., Griffin  J. D., Tedder  T. F.
ISSN
0887-6924 (Print)
Statut éditorial
Publié
Date de publication
04/1991
Volume
5
Numéro
4
Pages
300-308
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
The human leukocyte adhesion molecule-1 (LAM-1, TQ1, Leu-8) is involved in the binding of human leukocytes to high endothelial venules (HEV) of peripheral lymph nodes (LN). The regulation of LAM-1 expression is unique in that leukocyte stimulation induces a rapid down-modulation of LAM-1 from the cell surface. In this study, the regulation and function of LAM-1 was studied in detail in normal lymphocytes and compared with the LAM-1 of malignant leukocytes. Modulation of LAM-1 from the cell surface occurred concomitantly with the appearance of LAM-1 in the culture medium indicating that LAM-1 is cleaved from the cell surface. Shedding of LAM-1 was decreased in the presence of protein kinase C (PKC) inhibitors. As with normal lymphocytes, cells transfected with the LAM-1 cDNA and chronic lymphocytic leukemia (CLL) cells also shed LAM-1 following phorbol myristate acetate (PMA) exposure. CLL cells expressed the same Mr LAM-1 protein as normal lymphocytes and LAM-1+ CLL cells were able to specifically bind to HEV. In addition, normal lymphocytes and LAM-1+ CLL cells were capable of binding polyphosphomonester core polysaccharide (PPME) derived from yeast cell wall, a carbohydrate which mimics an essential component of the natural ligand for LAM-1, and PPME and HEV binding was specifically blocked by a new monoclonal antibody (mAb) reactive with LAM-1. The expression of LAM-1 and other adhesion molecules was examined on cells of 118 hematopoietic malignancies. LAM-1 was most frequently expressed on CLL and follicular or diffuse small cleaved cell lymphomas, whereas most other malignancies were LAM-1-. Thus, most CLL cells and some non-Hodgkin's lymphoma cells express a functionally active LAM-1 molecule which may correlate with their capacity to migrate through the circulation and disseminate into peripheral LN.
Mots-clé
Cell Adhesion Molecules/metabolism/*physiology Down-Regulation/physiology Fluorescent Antibody Technique Humans L-Selectin Leukemia/*blood Leukemia, Lymphocytic, Chronic/blood Leukocytes, Mononuclear/metabolism/*physiology Precipitin Tests Receptors, Leukocyte-Adhesion/physiology Tetradecanoylphorbol Acetate/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 15:31
Dernière modification de la notice
20/08/2019 12:25
Données d'usage