Characterization by DNA restriction endonuclease analysis of Listeria monocytogenes strains related to the Swiss epidemic of listeriosis.

Détails

ID Serval
serval:BIB_02ECBBA3B8E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Characterization by DNA restriction endonuclease analysis of Listeria monocytogenes strains related to the Swiss epidemic of listeriosis.
Périodique
Journal of Clinical Microbiology
Auteur(s)
Nocera D., Bannerman E., Rocourt J., Jaton-Ogay K., Bille J.
ISSN
0095-1137 (Print)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
1990
Peer-reviewed
Oui
Volume
28
Numéro
10
Pages
2259-2263
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Listeria monocytogenes strains responsible for outbreaks of listeriosis were studied by using serotyping and phage typing. An additional approach based on restriction endonuclease analysis (REA) of the chromosomal DNA was used to characterize L. monocytogenes strains collected from various sources during and after a Swiss outbreak of listeriosis (1983 to 1987). Among the 169 wild-type strains of Listeria spp. that were examined, 161 (95%) belonged to the species L. monocytogenes, of which 109 were of human origin. Ten different REA profiles were obtained from the 120 L. monocytogenes serotype 4b strains tested. All 57 serotype 4b strains that were identified as Swiss epidemic strains by phage typing clustered in two closely related REA profiles. In particular, 10 L. monocytogenes 4b strains isolated from the brand of soft cheese responsible for the outbreak and from its direct environment were indistinguishable from isolates from 40 patients by both phage typing and REA analysis. However, 5 of the 17 non-phage-typeable L. monocytogenes strains and 18 L. monocytogenes strains with a phage type different from those of the Swiss epidemic types showed the same profile. REA enabled the characterization of non-phage-typeable strains and, thus, seems a promising tool for L. monocytogenes typing, especially during epidemiological investigations.
Mots-clé
Bacteriophages/classification, DNA Restriction Enzymes, DNA, Bacterial/genetics, Disease Outbreaks, Evaluation Studies as Topic, Humans, Listeria monocytogenes/classification, Listeria monocytogenes/genetics, Listeriosis/epidemiology, Listeriosis/microbiology, Serotyping, Switzerland/epidemiology
Pubmed
Web of science
Création de la notice
11/02/2008 12:39
Dernière modification de la notice
20/08/2019 12:25
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