The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta.

Détails

ID Serval
serval:BIB_026D9182BA59
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta.
Périodique
Molecular Cell
Auteur⸱e⸱s
Martinon F., Burns K., Tschopp J.
ISSN
1097-2765 (Print)
ISSN-L
1097-2765
Statut éditorial
Publié
Date de publication
2002
Volume
10
Numéro
2
Pages
417-426
Langue
anglais
Résumé
Generation of Interleukin (IL)-1beta via cleavage of its proform requires the activity of caspase-1 (and caspase-11 in mice), but the mechanism involved in the activation of the proinflammatory caspases remains elusive. Here we report the identification of a caspase-activating complex that we call the inflammasome. The inflammasome comprises caspase-1, caspase-5, Pycard/Asc, and NALP1, a Pyrin domain-containing protein sharing structural homology with NODs. Using a cell-free system, we show that proinflammatory caspase activation and proIL-1beta processing is lost upon prior immunodepletion of Pycard. Moreover, expression of a dominant-negative form of Pycard in differentiated THP-1 cells blocks proIL-1beta maturation and activation of inflammatory caspases induced by LPS in vivo. Thus, the inflammasome constitutes an important arm of the innate immunity.
Mots-clé
Adaptor Proteins, Signal Transducing, Apoptosis Regulatory Proteins, Blotting, Western, Caspase 1/metabolism, Caspases/metabolism, Cell Line, Cell-Free System, Cytoskeletal Proteins/genetics, Cytoskeletal Proteins/metabolism, Enzyme Activation, Hela Cells, Humans, Inflammation/enzymology, Inflammation/metabolism, Interleukin-1/metabolism, Lipopolysaccharides/pharmacology, Macromolecular Substances, Protein Precursors/metabolism, Protein Processing, Post-Translational/drug effects, Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:19
Dernière modification de la notice
20/08/2019 12:24
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