Monocyte differentiation toward regulatory dendritic cells is not affected by respiratory syncytial virus-induced inflammatory mediators.

Détails

ID Serval
serval:BIB_01EADAA6D340
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Monocyte differentiation toward regulatory dendritic cells is not affected by respiratory syncytial virus-induced inflammatory mediators.
Périodique
American Journal of Respiratory Cell and Molecular Biology
Auteur⸱e⸱s
Sluijs K.F., Obregon C., Geiser T.K., Mühlemann K., Nicod L.P.
ISSN
1535-4989 (Electronic)
ISSN-L
1044-1549
Statut éditorial
Publié
Date de publication
2011
Volume
44
Numéro
5
Pages
655-664
Langue
anglais
Résumé
Airway epithelial cells were shown to drive the differentiation of monocytes into dendritic cells (DCs) with a suppressive phenotype. In this study, we investigated the impact of virus-induced inflammatory mediator production on the development of DCs. Monocyte differentiation into functional DCs, as reflected by the expression of CD11c, CD123, BDCA-4, and DC-SIGN and the capacity to activate T cells, was similar for respiratory syncytial virus (RSV)-infected and mock-infected BEAS-2B and A549 cells. RSV-conditioned culture media resulted in a partially mature DC phenotype, but failed to up-regulate CD80, CD83, CD86, and CCR7, and failed to release proinflammatory mediators upon Toll-like receptor (TLR) triggering. Nevertheless, these DCs were able to maintain an antiviral response by the release of Type I IFN. Collectively, these data indicate that the airway epithelium maintains an important suppressive DC phenotype under the inflammatory conditions induced by infection with RSV.
Mots-clé
Antigens, CD/biosynthesis, Antigens, CD80/biosynthesis, Antigens, CD86/biosynthesis, Cell Line, Tumor, Dendritic Cells/cytology, Epithelial Cells/cytology, Flow Cytometry/methods, Humans, Immunoglobulins/biosynthesis, Inflammation/metabolism, Inflammation Mediators/metabolism, Membrane Glycoproteins/biosynthesis, Monocytes/cytology, Monocytes/immunology, Phenotype, Receptors, CCR7/biosynthesis, Respiratory Syncytial Viruses/metabolism, Toll-Like Receptors/metabolism
Pubmed
Création de la notice
11/03/2011 10:58
Dernière modification de la notice
20/08/2019 12:24
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