Longitudinal evaluation of multiple biomarkers for the detection of testosterone gel administration in women with normal menstrual cycle.

Détails

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Etat: Public
Version: Final published version
Licence: Tous droits réservés
ID Serval
serval:BIB_0158257BBCEA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Longitudinal evaluation of multiple biomarkers for the detection of testosterone gel administration in women with normal menstrual cycle.
Périodique
Drug testing and analysis
Auteur(s)
Salamin O., Nicoli R., Langer T., Boccard J., Grundisch C.S., Xu C., Rudaz S., Kuuranne T., Pitteloud N., Saugy M.
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Statut éditorial
Publié
Date de publication
05/04/2021
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Résumé
In women, hormonal fluctuations related to the menstrual cycle may impose a great source of variability for some biomarkers of testosterone (T) administration, which can ultimately disrupt the sensitivity of their longitudinal monitoring. In this study, the sensitivity of the current urinary and haematological markers of the Athlete Biological Passport (ABP), as well as serum steroid biomarkers, was investigated for the monitoring of a 28-day T gel treatment combined with endogenous fluctuation of the menstrual cycle in 14 healthy female subjects. Additionally, the analysis of urinary target compounds was performed on a subset of samples for endogenous/exogenous origin via isotope ratio mass spectrometry (IRMS). In serum, concentrations of T and dihydrotestosterone (DHT) increased significantly during the treatment, whereas in urine matrix the most affected biomarkers were found to be the ratios of testosterone/epitestosterone (T/E) and 5α-androstane-3α,17β-diol/epitestosterone (5αAdiol/E). The detection capability of both urinary biomarkers was heavily influenced by [E], which fluctuated depending on the menstrual cycle, and resulted in low sensitivity of the urinary steroidal ABP module. On the contrary, an alternative approach by the longitudinal monitoring of serum T and DHT concentrations with the newly proposed T/androstenedione ratio showed higher sensitivity. The confirmatory IRMS results demonstrated that less than one third of the tested urine samples fulfilled the criteria for positivity. Results from this study demonstrated that the 'blood steroid profile' represents a powerful complementary approach to the 'urinary module' and underlines the importance of gathering bundle of evidence to support the scenario of an endogenous prohibited substance administration.
Mots-clé
Athlete Biological Passport, menstrual cycle, steroid profile, testosterone gel, women
Pubmed
Web of science
Création de la notice
06/04/2021 13:15
Dernière modification de la notice
26/05/2021 6:35
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