A Network of Paralogous Stress Response Transcription Factors in the Human Pathogen Candida Glabrata

Détails

Ressource 1Télécharger: Merhej et al. - 2016 - A Network of Paralogous Stress Response Transcript.pdf (3509.30 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_014BE8A86387
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A Network of Paralogous Stress Response Transcription Factors in the Human Pathogen Candida Glabrata
Périodique
Frontiers in Microbiology
Auteur(s)
Merhej Jawad, Thiébaut Antonin, Blugeon Corinne, Pouch Juliette, Chaouche Ali, Amine Mohammed El, Camadro Jean-Michel, Le Crom Stéphane, Lelandais Gaëlle, Devaux Frédéric
ISSN
1664-302X
Statut éditorial
Publié
Date de publication
2016
Volume
7
Langue
anglais
Résumé
The yeast Candida glabrata has become the second cause of systemic candidemia in humans. However, relatively few genome-wide studies have been conducted in this organism and our knowledge of its transcriptional regulatory network is quite limited. In the present work, we combined genome-wide chromatin immunoprecipitation (ChIP-seq), transcriptome analyses and DNA binding motif predictions to describe the regulatory interactions of the seven Yap (Yeast AP1) transcription factors of C. glabrata. We described a transcriptional network containing 255 regulatory interactions and 309 potential target genes. We predicted with high confidence the preferred DNA binding sites for 5 of the 7 CgYaps and showed a strong conservation of the Yap DNA binding properties between S. cerevisiae and C. glabrata. We provided reliable functional annotation for 3 of the 7 Yaps and identified for Yap1 and Yap5 a core regulon which is conserved in S. cerevisiae, C. glabrata and C. albicans. We uncovered new roles for CgYap7 in the regulation of iron-sulfur cluster biogenesis, for CgYap1 in the regulation of heme biosynthesis and for CgYap5 in the repression of GRX4 in response to iron starvation. These transcription factors define an interconnected transcriptional network at the cross-roads between redox homeostasis, oxygen consumption and iron metabolism.
Mots-clé
yeast,regulatory networks,ChIP-seq,evolution,transciptome,transcriptome,Yap,Yap proteins
Création de la notice
22/01/2020 14:58
Dernière modification de la notice
23/01/2020 7:26
Données d'usage