Phylogenomics of Gesneriaceae using targeted capture of nuclear genes.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_0102B1359D1E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Phylogenomics of Gesneriaceae using targeted capture of nuclear genes.
Périodique
Molecular phylogenetics and evolution
Auteur(s)
Ogutcen Ezgi, Christe Camille, Nishii Kanae, Salamin Nicolas, Möller Michael, Perret Mathieu
ISSN
1095-9513 (Electronic)
ISSN-L
1055-7903
Statut éditorial
Publié
Date de publication
04/2021
Peer-reviewed
Oui
Volume
157
Pages
107068
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Gesneriaceae (ca. 3400 species) is a pantropical plant family with a wide range of growth form and floral morphology that are associated with repeated adaptations to different environments and pollinators. Although Gesneriaceae systematics has been largely improved by the use of Sanger sequencing data, our understanding of the evolutionary history of the group is still far from complete due to the limited number of informative characters provided by this type of data. To overcome this limitation, we developed here a Gesneriaceae-specific gene capture kit targeting 830 single-copy loci (776,754 bp in total), including 279 genes from the Universal Angiosperms-353 kit. With an average of 557,600 reads and 87.8% gene recovery, our target capture was successful across the family Gesneriaceae and also in other families of Lamiales. From our bait set, we selected the most informative 418 loci to resolve phylogenetic relationships across the entire Gesneriaceae family using maximum likelihood and coalescent-based methods. Upon testing the phylogenetic performance of our baits on 78 taxa representing 20 out of 24 subtribes within the family, we showed that our data provided high support for the phylogenetic relationships among the major lineages, and were able to provide high resolution within more recent radiations. Overall, the molecular resources we developed here open new perspectives for the study of Gesneriaceae phylogeny at different taxonomical levels and the identification of the factors underlying the diversification of this plant group.
Mots-clé
African violet, phylogenetics, probe design, sequence capture, systematics, target enrichment, Phylogenetics, Probe design, Sequence capture, Systematics, Target enrichment
Pubmed
Open Access
Oui
Création de la notice
25/01/2021 9:49
Dernière modification de la notice
08/07/2021 6:09
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