Insulin secretion is regulated by the glucose-dependent production of islet beta cell macrophage migration inhibitory factor.

Détails

ID Serval
serval:BIB_00FEA018BE42
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Insulin secretion is regulated by the glucose-dependent production of islet beta cell macrophage migration inhibitory factor.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
Waeber G., Calandra T., Roduit R., Haefliger J.A., Bonny C., Thompson N., Thorens B., Temler E., Meinhardt A., Bacher M., Metz C.N., Nicod P., Bucala R.
ISSN
0027-8424
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
94
Numéro
9
Pages
4782-4787
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Macrophage migration inhibitory factor (MIF), originally identified as a cytokine secreted by T lymphocytes, was found recently to be both a pituitary hormone and a mediator released by immune cells in response to glucocorticoid stimulation. We report here that the insulin-secreting beta cell of the islets of Langerhans expresses MIF and that its production is regulated by glucose in a time- and concentration-dependent manner. MIF and insulin colocalize by immunocytochemistry within the secretory granules of the pancreatic islet beta cells, and once released, MIF appears to regulate insulin release in an autocrine fashion. In perifusion studies performed with isolated rat islets, immunoneutralization of MIF reduced the first and second phase of the glucose-induced insulin secretion response by 39% and 31%, respectively. Conversely, exogenously added recombinant MIF was found to potentiate insulin release. Constitutive expression of MIF antisense RNA in the insulin-secreting INS-1 cell line inhibited MIF protein synthesis and decreased significantly glucose-induced insulin release. MIF is therefore a glucose-dependent, islet cell product that regulates insulin secretion in a positive manner and may play an important role in carbohydrate metabolism.
Mots-clé
Animals, COS Cells, Cell Line, Cytoplasmic Granules/chemistry, Gene Expression, Glucose/pharmacology, Immunohistochemistry, Insulin/secretion, Islets of Langerhans/drug effects, Islets of Langerhans/metabolism, Macrophage Migration-Inhibitory Factors/biosynthesis, Macrophage Migration-Inhibitory Factors/genetics, Mice, RNA, Antisense/pharmacology, RNA, Messenger/isolation &amp, purification, Rats
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 13:41
Dernière modification de la notice
20/08/2019 12:23
Données d'usage