Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria
Détails
ID Serval
serval:BIB_006B8D4A2ECE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Conversion by Peyer's patch lymphocytes of human enterocytes into M cells that transport bacteria
Périodique
Science
ISSN
0036-8075 (Print)
Statut éditorial
Publié
Date de publication
08/1997
Volume
277
Numéro
5328
Pages
949-52
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 15
Research Support, Non-U.S. Gov't --- Old month value: Aug 15
Résumé
The epithelium that lines the gut is impermeable to macromolecules and microorganisms, except in Peyer's patches (PPs), where the lymphoid follicle-associated epithelium (FAE) contains M cells that transport antigens and microorganisms. A cultured system that reproduces the main characteristics of FAE and M cells was established by cultivation of PP lymphocytes with the differentiated human intestinal cell line Caco-2. Lymphocytes settled into the epithelial monolayer, inducing reorganization of the brush border and a temperature-dependent transport of particles and Vibrio cholerae. This model system could prove useful for intestinal physiology, vaccine research, and drug delivery studies.
Mots-clé
Animals
B-Lymphocytes/immunology
*Bacterial Translocation
Caco-2 Cells
Carrier Proteins/analysis
Cell Polarity
Coculture Techniques
Fluorescein-5-isothiocyanate
Humans
*Immunity, Mucosal
Intestinal Mucosa/*cytology/*immunology/microbiology
Lymphocytes/*immunology
Mice
Mice, Inbred BALB C
Microfilament Proteins/analysis
Microspheres
Microvilli/chemistry/ultrastructure
Peyer's Patches/cytology/*immunology
Sucrase-Isomaltase Complex/analysis
T-Lymphocytes/immunology
Temperature
Vibrio cholerae/*metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 16:06
Dernière modification de la notice
20/08/2019 13:22