Serial in vivo MR tracking of magnetically labeled neural spheres transplanted in chronic EAE mice.

Détails

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Etat: Supprimée
Version: de l'auteur
ID Serval
serval:BIB_0067F22D0C50
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Serial in vivo MR tracking of magnetically labeled neural spheres transplanted in chronic EAE mice.
Périodique
Magnetic Resonance In Medicine : Official Journal of the Society of Magnetic Resonance In Medicine / Society of Magnetic Resonance In Medicine
Auteur(s)
Ben-Hur T., van Heeswijk R.B., Einstein O., Aharonowiz M., Xue R., Frost E.E., Mori S., Reubinoff B.E., Bulte J.W.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
2007
Volume
57
Numéro
1
Pages
164-171
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
Neural stem cell (NSC) transplantation has been shown to attenuate the severity of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Central to the future success of NSC transplantation in MS is the ability of transplanted cells to migrate from the site of transplantation to relevant foci of disease. Using magnetically labeled mouse neurospheres and human embryonic stem cell (hESC)-derived neurospheres, we applied serial magnetic resonance imaging (MRI) to assess the biodynamics of transplanted cell migration in a chronic mouse EAE model. Magnetic labeling did not affect the in vitro and in vivo characteristics of cells as multipotential precursors. Cell migration occurred along white matter (WM) tracts (especially the corpus callosum (CC), fimbria, and internal capsule), predominantly early in the acute phase of disease, and in an asymmetric manner. The distance of cell migration correlated well with clinical severity of disease and the number of microglia in the WM tracts, supporting the notion that inflammatory signals promote transplanted cell migration. This study shows for the first time that hESC-derived neural precursors also respond to tissue signals in an MS model, similarly to rodent cells. The results are directly relevant for designing and optimizing cell therapies for MS, and achieving a better understanding of in vivo cell dynamics and cell-tissue interactions.
Mots-clé
Animals, Cell Lineage, Cell Movement, Cells, Cultured, Disease Models, Animal, Encephalomyelitis, Autoimmune, Experimental/chemically induced, Encephalomyelitis, Autoimmune, Experimental/physiopathology, Humans, Magnetic Resonance Imaging, Mice, Mice, Inbred C57BL, Neurons/cytology, Neurons/physiology, Severity of Illness Index, Spheroids, Cellular/cytology, Spheroids, Cellular/transplantation, Stem Cell Transplantation, Transplantation, Heterologous
Pubmed
Open Access
Oui
Création de la notice
13/12/2012 17:31
Dernière modification de la notice
20/08/2019 13:22
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