Distribution of the human intracellular serpin protease inhibitor 8 in human tissues.

Détails

ID Serval
serval:BIB_0037E512BAB6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Distribution of the human intracellular serpin protease inhibitor 8 in human tissues.
Périodique
Journal of Histochemistry and Cytochemistry
Auteur(s)
Strik M.C., Bladergroen B.A., Wouters D., Kisiel W., Hooijberg J.H., Verlaan A.R., Hordijk P.L., Schneider P., Hack C.E., Kummer J.A.
ISSN
0022-1554 (Print)
ISSN-L
0022-1554
Statut éditorial
Publié
Date de publication
2002
Volume
50
Numéro
11
Pages
1443-1454
Langue
anglais
Résumé
Ovalbumin-like serine protease inhibitors are mainly localized intracellularly and their in vivo functions are largely unknown. To elucidate their physiological role(s), we studied the expression of one of these inhibitors, protease inhibitor 8 (PI-8), in normal human tissues by immunohistochemistry using a PI-8-specific monoclonal antibody. PI-8 was strongly expressed in the nuclei of squamous epithelium of mouth, pharynx, esophagus, and epidermis, and by the epithelial layer of skin appendages, particularly by more differentiated epithelial cells. PI-8 was also expressed by monocytes and by neuroendocrine cells in the pituitary gland, pancreas, and digestive tract. Monocytes showed nuclear and cytoplasmic localization of PI-8, whereas neuroendocrine cells showed only cytoplasmic staining. In vitro nuclear localization of PI-8 was confirmed by confocal analysis using serpin-transfected HeLa cells. Furthermore, mutation of the P(1) residue did not affect the subcellular distribution pattern of PI-8, indicating that its nuclear localization is independent of the interaction with its target protease. We conclude that PI-8 has a unique distribution pattern in human tissues compared to the distribution patterns of other intracellular serpins. Additional studies must be performed to elucidate its physiological role.
Mots-clé
Animals, Antibodies, Monoclonal, COS Cells, Cell Nucleus/metabolism, Epithelial Cells/metabolism, Epithelial Cells/ultrastructure, Humans, Immunohistochemistry, Intracellular Fluid/metabolism, Monocytes/metabolism, Neurosecretory Systems/cytology, Neurosecretory Systems/metabolism, Organ Specificity, Serpins/immunology, Serpins/metabolism, Subcellular Fractions/metabolism
Pubmed
Web of science
Création de la notice
19/01/2008 18:30
Dernière modification de la notice
20/08/2019 13:22
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