Cultured human fetal astrocytes can be induced by interferon-gamma to express HLA-DR.

Détails

ID Serval
serval:BIB_001E2FDEA7EB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cultured human fetal astrocytes can be induced by interferon-gamma to express HLA-DR.
Périodique
Journal of neuroimmunology
Auteur(s)
Pulver M., Carrel S., Mach J.P., de Tribolet N.
ISSN
0165-5728
Statut éditorial
Publié
Date de publication
1987
Peer-reviewed
Oui
Volume
14
Numéro
2
Pages
123-133
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Interferon-gamma (IFN-gamma) modulates the expression of Class II major histocompatibility antigens (MHC), thus providing a potential regulatory mechanism for local immune reactivity in the context of MHC-restricted antigen presentation. Within the central nervous system (CNS), the expression of MHC Class II antigens has been demonstrated on human reactive astrocytes and glioma cells. In order to investigate the modulation of HLA-DR on normal astrocytes, two cell lines were grown from a 20-week-old fetal brain. In situ none of the fetal brain cells expressed HLA-DR as determined by immunohistology on frozen tissue sections. The two cell lines, FB I and FB II, expressed GFAP indicating their astrocytic origin. FB I was HLA-DR negative at the first tissue culture passages, but could be induced to express HLA-DR when treated with 500 U/ml IFN-gamma. FB II was spontaneously HLA-DR positive in the early passages, lost the expression of this antigen after 11 passages and could also be induced to express HLA-DR by IFN-gamma. The induction of HLA-DR expression was demonstrated both by a binding RIA and by immunoprecipitation using a monoclonal antibody (MAB) directed against a monomorphic determinant of HLA-DR. The HLA-DR alloantigens were determined on FB II cells after IFN-gamma treatment, by immunofluorescence and by cytotoxicity assays, and were shown to be DR4, DR6, Drw52, DRw53 and DQwl. These results show that human fetal astrocytes can be induced to express HLA-DR by IFN-gamma in vitro and support the concept that astrocytes may function as antigen-presenting cells.
Mots-clé
Astrocytes/immunology, Brain/cytology, Brain/embryology, Cell Line, Fetus/cytology, Glial Fibrillary Acidic Protein/analysis, HLA-D Antigens/immunology, HLA-DR Antigens/immunology, Humans, Interferon-gamma/pharmacology, Phenotype, Recombinant Proteins/pharmacology
Pubmed
Web of science
Création de la notice
19/11/2009 13:37
Dernière modification de la notice
20/08/2019 13:22
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