Lipopolysaccharide (LPS)-binding protein in human serum determines the tumor necrosis factor response of monocytes to LPS

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Serval ID
serval:BIB_FF9B9DB65BA8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lipopolysaccharide (LPS)-binding protein in human serum determines the tumor necrosis factor response of monocytes to LPS
Journal
Journal of Infectious Diseases
Author(s)
Gallay P., Barras C., Tobias P. S., Calandra T., Glauser M. P., Heumann D.
ISSN
0022-1899 (Print)
Publication state
Published
Issued date
11/1994
Volume
170
Number
5
Pages
1319-22
Language
english
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
Lipopolysaccharide (LPS)-binding protein (LBP) and CD14 represent key elements in monocyte activation by LPS. The mean concentration of LBP was 18.1 microgram/mL in normal serum and 40-60 micrograms/mL in serum of patients with septic shock, independent of the fact that patients had gram-negative or other infections. Ten percent normal serum presented large concentrations of LPS (in the microgram range) to monocytes. Only when diluted 1:100 was LBP in plasma a limiting factor for monocyte activation, as measured by tumor necrosis factor (TNF) release. When LBP was depleted from serum with anti-LBP antibodies, the resulting serum did not support TNF release of monocytes upon LPS challenge. In conclusion, monocyte activation resulting in TNF secretion was related to LBP, which is abundantly present in normal serum, and elevated two to three times in patients with septic shock.
Keywords
*Acute-Phase Proteins Carrier Proteins/*blood/physiology Flow Cytometry Humans Lipopolysaccharides/metabolism/*pharmacology *Membrane Glycoproteins Monocytes/*drug effects/secretion Radioimmunoassay Tumor Necrosis Factor-alpha/*secretion
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:28
Last modification date
01/10/2019 7:20
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