PAX3/FOXO1 fusion gene status is the key prognostic molecular marker in rhabdomyosarcoma and significantly improves current risk stratification.

Details

Serval ID
serval:BIB_FF3A89135856
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PAX3/FOXO1 fusion gene status is the key prognostic molecular marker in rhabdomyosarcoma and significantly improves current risk stratification.
Journal
Journal of Clinical Oncology
Author(s)
Missiaglia E., Williamson D., Chisholm J., Wirapati P., Pierron G., Petel F., Concordet J.P., Thway K., Oberlin O., Pritchard-Jones K., Delattre O., Delorenzi M., Shipley J.
ISSN
1527-7755 (Electronic)
ISSN-L
0732-183X
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
30
Number
14
Pages
1670-1677
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Meta-Analysis ; Research Support, Non-U.S. Gov't
Abstract
PURPOSE: To improve the risk stratification of patients with rhabdomyosarcoma (RMS) through the use of clinical and molecular biologic data.
PATIENTS AND METHODS: Two independent data sets of gene-expression profiling for 124 and 101 patients with RMS were used to derive prognostic gene signatures by using a meta-analysis. These and a previously published metagene signature were evaluated by using cross validation analyses. A combined clinical and molecular risk-stratification scheme that incorporated the PAX3/FOXO1 fusion gene status was derived from 287 patients with RMS and evaluated.
RESULTS: We showed that our prognostic gene-expression signature and the one previously published performed well with reproducible and significant effects. However, their effect was reduced when cross validated or tested in independent data and did not add new prognostic information over the fusion gene status, which is simpler to assay. Among nonmetastatic patients, patients who were PAX3/FOXO1 positive had a significantly poorer outcome compared with both alveolar-negative and PAX7/FOXO1-positive patients. Furthermore, a new clinicomolecular risk score that incorporated fusion gene status (negative and PAX3/FOXO1 and PAX7/FOXO1 positive), Intergroup Rhabdomyosarcoma Study TNM stage, and age showed a significant increase in performance over the current risk-stratification scheme.
CONCLUSION: Gene signatures can improve current stratification of patients with RMS but will require complex assays to be developed and extensive validation before clinical application. A significant majority of their prognostic value was encapsulated by the fusion gene status. A continuous risk score derived from the combination of clinical parameters with the presence or absence of PAX3/FOXO1 represents a robust approach to improving current risk-adapted therapy for RMS.
Keywords
Adolescent, Child, Child, Preschool, Cohort Studies, Databases, Factual, Female, Forkhead Transcription Factors/genetics, Forkhead Transcription Factors/metabolism, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Gene Fusion/genetics, Great Britain, Humans, Kaplan-Meier Estimate, Male, Multivariate Analysis, Neoplasm Invasiveness/pathology, Neoplasm Staging, Paired Box Transcription Factors/genetics, Paired Box Transcription Factors/metabolism, Prognosis, Proportional Hazards Models, Retrospective Studies, Rhabdomyosarcoma/genetics, Rhabdomyosarcoma/mortality, Risk Management, Sensitivity and Specificity, Survival Analysis, Translocation, Genetic, Tumor Markers, Biological/genetics
Pubmed
Web of science
Create date
09/06/2012 19:03
Last modification date
20/08/2019 17:29
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