NMR microsystem for label-free characterization of 3D nanoliter microtissues.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_FED7749F0E2E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
NMR microsystem for label-free characterization of 3D nanoliter microtissues.
Journal
Scientific reports
Author(s)
Grisi M., Conley G.M., Rodriguez K.J., Riva E., Egli L., Moritz W., Lichtenberg J., Brugger J., Boero G.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
27/10/2020
Peer-reviewed
Oui
Volume
10
Number
1
Pages
18306
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Performing chemical analysis at the nanoliter (nL) scale is of paramount importance for medicine, drug development, toxicology, and research. Despite the numerous methodologies available, a tool for obtaining chemical information non-invasively is still missing at this scale. Observer effects, sample destruction and complex preparatory procedures remain a necessary compromise. Among non-invasive spectroscopic techniques, one able to provide holistic and highly resolved chemical information in-vivo is nuclear magnetic resonance (NMR). For its renowned informative power and ability to foster discoveries and life-saving applications, efficient NMR at microscopic scales is highly sought after, but so far technical limitations could not match the stringent necessities of microbiology, such as biocompatible handling, ease of use, and high throughput. Here we introduce a novel microsystem, which combines CMOS technology with 3D microfabrication, enabling nL NMR as a platform tool for non-invasive spectroscopy of organoids, 3D cell cultures, and early stage embryos. In this study we show its application to microlivers models simulating non-alcoholic fatty liver disease, demonstrating detection of lipid metabolism dynamics in a time frame of 14 days based on 117 measurements of single 3D human liver microtissues.
Pubmed
Web of science
Open Access
Yes
Create date
02/11/2020 12:38
Last modification date
08/08/2024 6:43
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