Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins

Details

Serval ID
serval:BIB_FE9D7FCC0CE6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins
Journal
Molecular Biology of the Cell
Author(s)
Bruzzone  R., Haefliger  J. A., Gimlich  R. L., Paul  D. L.
ISSN
1059-1524 (Print)
Publication state
Published
Issued date
01/1993
Volume
4
Number
1
Pages
7-20
Notes
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Abstract
The cellular distribution of connexin40 (Cx40), a newly cloned gap junction structural protein, was examined by immunofluorescence microscopy using two different specific anti-peptide antibodies. Cx40 was detected in the endothelium of muscular as well as elastic arteries in a punctate pattern consistent with the known distribution of gap junctions. However, it was not detected in other cells of the vascular wall. By contrast, Cx43, another connexin present in the cardiovascular system, was not detected in endothelial cells of muscular arteries but was abundant in the myocardium and aortic smooth muscle. We have tested the ability of these connexins to interact functionally. Cx40 was functionally expressed in pairs of Xenopus oocytes and induced the formation of intercellular channels with unique voltage dependence. Unexpectedly, communication did not occur when oocytes expressing Cx40 were paired with those expressing Cx43, although each could interact with a different connexin, Cx37, to form gap junction channels in paired oocytes. These findings indicate that establishment of intercellular communication can be spatially regulated by the selective expression of different connexins and suggest a mechanism that may operate to control the extent of communication between cells.
Keywords
Amino Acid Sequence Animals Base Sequence Cardiovascular System/metabolism Cell Communication/physiology Connexins DNA/genetics Electrochemistry Endothelium, Vascular/*metabolism/ultrastructure Fluorescent Antibody Technique Intercellular Junctions/*metabolism Membrane Proteins/genetics/immunology/*metabolism Molecular Sequence Data Oocytes/metabolism/ultrastructure Peptide Fragments/genetics/immunology Rats Xenopus
Pubmed
Web of science
Create date
25/01/2008 14:48
Last modification date
20/08/2019 17:29
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