Organization of the sex-ratio meiotic drive region in Drosophila simulans.

Details

Serval ID
serval:BIB_FE220983DA69
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Organization of the sex-ratio meiotic drive region in Drosophila simulans.
Journal
Genetics
Author(s)
Montchamp-Moreau C., Ogereau D., Chaminade N., Colard A., Aulard S.
ISSN
0016-6731
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
174
Number
3
Pages
1365-1371
Language
english
Abstract
Sex-ratio meiotic drive is the preferential transmission of the X chromosome by XY males, which occurs in several Drosophila species and results in female-biased progeny. Although the trait has long been known to exist, its molecular basis remains completely unknown. Here we report a fine-mapping experiment designed to characterize the major drive locus on a sex-ratio X chromosome of Drosophila simulans originating from the Seychelles (XSR6). This primary locus was found to contain two interacting elements at least, both of which are required for drive expression. One of them was genetically tracked to a tandem duplication containing six annotated genes (Trf2, CG32712, CG12125, CG1440, CG12123, org-1), and the other to a candidate region located approximately 110 kb away and spanning seven annotated genes. RT-PCR showed that all but two of these genes were expressed in the testis of both sex-ratio and standard males. In situ hybridization to polytene chromosomes revealed a complete association of the duplication with the sex-ratio trait in random samples of X chromosomes from Madagascar and Reunion.
Keywords
Animals, Base Sequence, Chromosomes, Drosophila/cytology, Drosophila/genetics, Female, Fluorescent Dyes, Gene Duplication, Genes, Insect, Genetic Markers, In Situ Hybridization, Indoles, Male, Meiosis, Molecular Sequence Data, Physical Chromosome Mapping, Recombination, Genetic, Sex Ratio, X Chromosome
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 10:56
Last modification date
20/08/2019 16:28
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