High-throughput identification of human antigen-specific CD8<sup>+</sup> and CD4<sup>+</sup> T cells using soluble pMHC multimers.

Details

Serval ID
serval:BIB_FD66E29F7790
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
High-throughput identification of human antigen-specific CD8<sup>+</sup> and CD4<sup>+</sup> T cells using soluble pMHC multimers.
Journal
Methods in enzymology
Author(s)
Magnin M., Guillaume P., Coukos G., Harari A., Schmidt J.
ISSN
1557-7988 (Electronic)
ISSN-L
0076-6879
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
631
Pages
21-42
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Peptide major histocompatibility complex (pMHC) multimers have been used since decades to identify, isolate and analyze antigen-specific T cells by flow (and more recently mass) cytometry. Yet well established as a standard technology, improvements are still required to face the growing needs of personalized immune monitoring. Here we review the latest developments about (i) the quality of pMHC class I and II monomers, (ii) the importance of the multimeric scaffold, (iii) the staining conditions and (iv) the high-throughput synthesis of pMHC monomers. Finally, innovative multiplexed, combinatorial strategies for parallel detection of antigen-specific T cells in a single sample are discussed.
Keywords
Antigen discovery, CD4(+) T cells, CD8(+) T cells, Combinatorial, Peptide-MHC multimers, Tetramers
Pubmed
Create date
23/01/2020 16:44
Last modification date
26/08/2020 6:26
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