Stability of K-ras mutations throughout the natural history of human colorectal cancer

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_FCB2A7DC4787
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stability of K-ras mutations throughout the natural history of human colorectal cancer
Journal
European Journal of Cancer
Author(s)
Losi L., Benhattar J., Costa J.
ISSN
0959-8049 (Print)
Publication state
Published
Issued date
1992
Volume
28A
Number
6-7
Pages
1115-1120
Language
english
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
We have used a rapid, non-radioactive and sensitive method based on allele-specific amplification using the polymerase chain reaction for the identification of K-ras mutations in archival tissues of colorectal carcinomas. Our purpose was to determine whether or not K-ras mutation provides, when present, a tumour marker throughout the natural history of the disease. We have studied 35 patients who developed recurrent cancer. In 71% of these patients a ras mutation in codons 12 or 13 was observed in the primary tumour. For each of these cases an identical ras mutation was found in the DNA from the local or distant recurrence. In the 29% of cases where no ras mutation was observed in the primary tumour, no newly acquired ras mutation appeared in the recurrent tumour. The time interval between primary tumours and recurrences varied from 3 to 60 months. Our results indicate that K-ras mutation provides a stable tumour marker throughout the natural history of colorectal cancer
Keywords
Adenocarcinoma/genetics/Aged/Aged,80 and over/Base Sequence/Colorectal Neoplasms/DNA,Neoplasm/analysis/Female/Genes,ras/Humans/Male/Middle Aged/Molecular Sequence Data/Mutation/Neoplasm Recurrence,Local/Polymerase Chain Reaction/methods/Tumor Markers,Biological/Research
Pubmed
Web of science
Create date
29/01/2008 19:35
Last modification date
27/06/2020 6:20
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