High-throughput monitoring of human tumor-specific T-cell responses with large peptide pools.

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State: Public
Version: Final published version
Serval ID
serval:BIB_FC441F50334F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High-throughput monitoring of human tumor-specific T-cell responses with large peptide pools.
Journal
Oncoimmunology
Author(s)
Chevalier M.F., Bobisse S., Costa-Nunes C., Cesson V., Jichlinski P., Speiser D.E., Harari A., Coukos G., Romero P., Nardelli-Haefliger D., Jandus C., Derré L.
ISSN
2162-4011 (Print)
ISSN-L
2162-4011
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
4
Number
10
Pages
e1029702
Language
english
Abstract
In immune intervention trials, the comprehensive investigation of immunogenicity or T-cell epitope-mapping is challenging especially when a large set of epitopes needs to be screened and limited sample material is available. To this end, T-cell responses are often monitored using peptide pools. Here, we assessed the magnitude and sensitivity of detection of antigen-specific CD8(+) and CD4(+) T cells using a single peptide alone or mixed into large pools. Interestingly the magnitude of ex vivo anti-viral and anti-tumor T-cell responses was identical irrespective of the presence and number of irrelevant peptides, in different functional assays with PBMCs from healthy donors and cancer patients. Moreover, the presence of up to 300 irrelevant peptides did not affect the threshold of responsiveness of antigen-specific CD8(+) T cells to single cognate peptides. These data demonstrate the relevance of using very large peptide pools for the sensitive and specific immune-monitoring of epitope-specific T cells in natural or immune-modulated context.
Pubmed
Create date
08/01/2016 12:45
Last modification date
20/08/2019 17:27
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