Maf1, a new player in the regulation of human RNA polymerase III transcription.

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Serval ID
serval:BIB_FC2177747AE6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Maf1, a new player in the regulation of human RNA polymerase III transcription.
Journal
PLoS ONE
Author(s)
Reina J.H., Azzouz T.N., Hernandez N.
ISSN
1932-6203
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
1
Pages
e134
Language
english
Notes
Publication types: Journal Article
Abstract
BACKGROUND: Human RNA polymerase III (pol III) transcription is regulated by several factors, including the tumor suppressors P53 and Rb, and the proto-oncogene c-Myc. In yeast, which lacks these proteins, a central regulator of pol III transcription, called Maf1, has been described. Maf1 is required for repression of pol III transcription in response to several signal transduction pathways and is broadly conserved in eukaryotes. METHODOLOGY/PRINCIPAL FINDINGS: We show that human endogenous Maf1 can be co-immunoprecipitated with pol III and associates in vitro with two pol III subunits, the largest subunit RPC1 and the alpha-like subunit RPAC2. Maf1 represses pol III transcription in vitro and in vivo and is required for maximal pol III repression after exposure to MMS or rapamycin, treatments that both lead to Maf1 dephosphorylation. CONCLUSIONS/SIGNIFICANCE: These data suggest that Maf1 is a major regulator of pol III transcription in human cells.
Pubmed
Web of science
Open Access
Yes
Create date
21/01/2008 17:33
Last modification date
20/08/2019 17:27
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