Transition to 37°C reveals importance of NADPH in mitigating oxidative stress in stored RBCs.

Details

Serval ID
serval:BIB_FC0F634C4BC0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transition to 37°C reveals importance of NADPH in mitigating oxidative stress in stored RBCs.
Journal
JCI insight
Author(s)
Roch A., Magon N.J., Maire J., Suarna C., Ayer A., Waldvogel S., Imhof B.A., Koury M.J., Stocker R., Schapira M.
ISSN
2379-3708 (Electronic)
ISSN-L
2379-3708
Publication state
Published
Issued date
01/11/2019
Peer-reviewed
Oui
Volume
4
Number
21
Pages
e126376
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The RBC storage lesion is a multiparametric response that occurs during storage at 4°C, but its impact on transfused patients remains unclear. In studies of the RBC storage lesion, the temperature transition from cold storage to normal body temperature that occurs during transfusion has received limited attention. We hypothesized that multiple deleterious events might occur in this period of increasing temperature. We show dramatic alterations in several properties of therapeutic blood units stored at 4°C after warming them to normal body temperature (37°C), as well as febrile temperature (40°C). In particular, the intracellular content and redox state of NADP(H) were directly affected by post-storage incubation at 37°C, as well as by pro-oxidant storage conditions. Modulation of the NADPH-producing pentose phosphate pathway, but not the prevention of hemoglobin autoxidation by conversion of oxyhemoglobin to carboxyhemoglobin, provided protection against storage-induced alterations in RBCs, demonstrating the central role of NADPH in mitigating increased susceptibility of stored RBCs to oxidative stress. We propose that assessing RBC oxidative status after restoration of body temperature constitutes a sensitive method for detecting storage-related alterations that has the potential to improve the quality of stored RBCs for transfusion.
Keywords
Cell stress, Hematology, Metabolism, Radicals
Pubmed
Web of science
Open Access
Yes
Create date
07/01/2020 13:02
Last modification date
21/05/2021 6:35
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