Cyclin A is a mediator of p120E4F-dependent cell cycle arrest in G1.
Details
Serval ID
serval:BIB_FB5186987042
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cyclin A is a mediator of p120E4F-dependent cell cycle arrest in G1.
Journal
Molecular and Cellular Biology
ISSN
0270-7306 (Print)
ISSN-L
0270-7306
Publication state
Published
Issued date
2001
Volume
21
Number
8
Pages
2956-2966
Language
english
Abstract
E4F is a ubiquitously expressed GLI-Krüppel-related transcription factor which has been identified for its capacity to regulate transcription of the adenovirus E4 gene in response to E1A. However, cellular genes regulated by E4F are still unknown. Some of these genes are likely to be involved in cell cycle progression since ectopic p120E4F expression induces cell cycle arrest in G1. Although p21WAF1 stabilization was proposed to mediate E4F-dependent cell cycle arrest, we found that p120E4F can induce a G1 block in p21(-/-) cells, suggesting that other proteins are essential for the p120E4F-dependent block in G1. We show here that cyclin A promoter activity can be repressed by p120E4F and that this repression correlates with p120E4F binding to the cyclic AMP-responsive element site of the cyclin A promoter. In addition, enforced expression of cyclin A releases p120E4F-arrested cells from the G1 block. These data identify the cyclin A gene as a cellular target for p120E4F and suggest a mechanism for p120E4F-dependent cell cycle regulation.
Keywords
3T3 Cells, Animals, Base Sequence, Binding Sites/genetics, Cell Line, Cricetinae, Cyclin A/genetics, Cyclin A/metabolism, Cyclin-Dependent Kinase Inhibitor p21, Cyclins/genetics, Cyclins/metabolism, DNA/genetics, DNA/metabolism, DNA Primers/genetics, G1 Phase/physiology, GA-Binding Protein Transcription Factor, Gene Expression, Mice, Mice, Knockout, Promoter Regions, Genetic, Repressor Proteins/genetics, Repressor Proteins/metabolism, Retinoblastoma Protein/genetics, Retinoblastoma Protein/metabolism, Signal Transduction, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Open Access
Yes
Create date
07/03/2013 17:08
Last modification date
20/08/2019 17:26