Analysis of parapoxvirus genomes.

Details

Serval ID
serval:BIB_F9D0C0AA66F6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of parapoxvirus genomes.
Journal
Archives of Virology
Author(s)
Gassmann U., Wyler R., Wittek R.
ISSN
0304-8608[print], 0304-8608[linking]
Publication state
Published
Issued date
1985
Volume
83
Number
1-2
Pages
17-31
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Eight stomatitis papulosa (SP), four orf and two milker's nodes (MN) virus isolates were compared by restriction enzyme analysis. Considerable genetic heterogeneity was found not only between isolates belonging to the three different taxonomic groups but also between members of the same group. This heterogeneity precludes classification of parapoxviruses simply by comparison of their DNA cleavage patterns. Restriction maps were therefore prepared for 12 parapoxvirus DNAs. Fragments from defined regions of the genome were then selected and used as probes for cross-hybridizations to all other parapoxvirus DNAs. DNA fragments derived from an internal region of the genome hybridized strongly to all parapoxvirus isolates examined. In contrast, cross-hybridization of the end region of the DNA molecule was observed only between members of the same virus group. Molecular hybridization as a means of classifying parapoxvirus isolates is discussed.
Keywords
DNA Restriction Enzymes, DNA, Viral/genetics, Electrophoresis, Agar Gel, Genes, Viral, Nucleic Acid Hybridization, Orf virus/genetics, Phylogeny, Poxviridae/classification, Poxviridae/genetics, Pseudocowpox virus/genetics
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 16:25
Usage data