Immunophotodiagnosis of colon carcinomas in patients injected with fluoresceinated chimeric antibodies against carcinoembryonic antigen

Details

Serval ID
serval:BIB_F9CDB49AA00D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immunophotodiagnosis of colon carcinomas in patients injected with fluoresceinated chimeric antibodies against carcinoembryonic antigen
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Folli  S., Wagnieres  G., Pelegrin  A., Calmes  J. M., Braichotte  D., Buchegger  F., Chalandon  Y., Hardman  N., Heusser  C., Givel  J. C., Chapuis  G., Chatelain  A., Vandenbergh  H., Mach  J. P.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
09/1992
Volume
89
Number
17
Pages
7973-7
Notes
Clinical Trial Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Abstract
Based on previous experiments in nude mice, showing that fluoresceinated monoclonal antibodies against carcinoembryonic antigen localized specifically in human carcinoma xenografts and could be detected by laser-induced fluorescence, we performed a feasibility study to determine whether this immunophotodiagnosis method could be applied in the clinic. Six patients, with known primary colorectal carcinoma, received an i.v. injection of 4.5 or 9 mg of mouse-human chimeric anti-carcinoembryonic antigen monoclonal antibody coupled with 0.10-0.28 mg of fluorescein (molar ratio 1/10 to 1/14). The monoclonal antibody was also labeled with 0.2-0.4 mCi of 125I (1 Ci = 37 GBq). Photodetection of the tumor was done ex vivo on surgically resected tissues for the six patients and in vivo by fluorescence rectosigmoidoscopy for the sixth patient. Upon laser irradiation, clearly detectable heterogeneous green fluorescence from the dye-antibody conjugate was visually observed on all six tumors; almost no such fluorescence was detectable on normal mucosa. The yellowish tissue autofluorescence, which was emitted from both tumor and normal mucosa, could be subtracted by real-time image processing. Radioactivity measurements confirmed the specificity of tumor localization by the conjugate; tissue concentrations of up to 0.059% injected dose per g of tumor and 10 times less (0.006%) per g of normal mucosa were found. The overall results demonstrate the feasibility of tumor immunophotodiagnosis at the clinical level.
Keywords
Antibody Affinity Carcinoembryonic Antigen/*analysis/immunology Carcinoma/*diagnosis Colorectal Neoplasms/*diagnosis Fluorescent Antibody Technique Humans Recombinant Fusion Proteins
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 8:56
Last modification date
20/08/2019 16:25
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