Human Induced Pluripotent Stem Cell-Derived Astrocytes Are Differentially Activated by Multiple Sclerosis-Associated Cytokines.

Details

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State: Public
Version: author
License: CC BY 4.0
Serval ID
serval:BIB_F9677A578D24
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human Induced Pluripotent Stem Cell-Derived Astrocytes Are Differentially Activated by Multiple Sclerosis-Associated Cytokines.
Journal
Stem cell reports
Author(s)
Perriot S., Mathias A., Perriard G., Canales M., Jonkmans N., Merienne N., Meunier C., El Kassar L., Perrier A.L., Laplaud D.A., Schluep M., Déglon N., Du Pasquier R.
ISSN
2213-6711 (Electronic)
ISSN-L
2213-6711
Publication state
Published
Issued date
13/11/2018
Peer-reviewed
Oui
Volume
11
Number
5
Pages
1199-1210
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Recent studies highlighted the importance of astrocytes in neuroinflammatory diseases, interacting closely with other CNS cells but also with the immune system. However, due to the difficulty in obtaining human astrocytes, their role in these pathologies is still poorly characterized. Here, we develop a serum-free protocol to differentiate human induced pluripotent stem cells (hiPSCs) into astrocytes. Gene expression and functional assays show that our protocol consistently yields a highly enriched population of resting mature astrocytes across the 13 hiPSC lines differentiated. Using this model, we first highlight the importance of serum-free media for astrocyte culture to generate resting astrocytes. Second, we assess the astrocytic response to IL-1β, TNF-α, and IL-6, all cytokines important in neuroinflammation, such as multiple sclerosis. Our study reveals very specific profiles of reactive astrocytes depending on the triggering stimulus. This model provides ideal conditions for in-depth and unbiased characterization of astrocyte reactivity in neuroinflammatory conditions.
Keywords
Astrocytes/drug effects, Astrocytes/metabolism, Astrocytes/pathology, Case-Control Studies, Cell Differentiation/drug effects, Cells, Cultured, Culture Media, Serum-Free, Cytokines/pharmacology, Humans, Induced Pluripotent Stem Cells/pathology, Inflammation Mediators/metabolism, Multiple Sclerosis/genetics, Multiple Sclerosis/pathology, Phenotype, Remyelination/drug effects, Transcriptome/drug effects, Transcriptome/genetics, astrocytes, differentiation, induced pluripotent stem cells, multiple sclerosis, neuroinflammation
Pubmed
Web of science
Open Access
Yes
Create date
12/11/2018 15:44
Last modification date
19/06/2020 6:21
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