Regulatory properties of pyridine-nucleotide transhydrogenase from pseudomonas-aeruginosa. Kinetic studies and fluorescence titration

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State: Public
Version: author
Serval ID
serval:BIB_F6BF028607ED
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulatory properties of pyridine-nucleotide transhydrogenase from pseudomonas-aeruginosa. Kinetic studies and fluorescence titration
Journal
Biochemistry
Author(s)
Widmer F., Kaplan N.O.
ISSN
0006-2960
Publication state
Published
Issued date
1976
Peer-reviewed
Oui
Volume
15
Number
21
Pages
4693-4699
Language
english
Abstract
Mechanisms involved in the action of the pyridine nucleotide transhydrogenase from Pseudomonas aeruginosa (EC 1.6.1.1) have been investigated by means of kinetic studies and fluorescence titration. Our results, as well as those from previous investigations, suggest that the allosteric MWC model (Monod, J., Wyman, J., and Changeux, J. P. (1965), J. Mol. Biol. 12, 88-118) may be used as a first step for the explanation of the properties of the transhydrogenase. The basic reaction of the enzyme is the oxidation of reduced triphosphopyridine nucleotide (TPNH) by diphosphopyridine nucleotide (DPN+). In terms of the model, the functional R state is favored by TPNH, whereas the product triphosphopyridine nucleotide (TPN+) behaves as an allosteric inhibitor, and is therefore assumed to favor the nonfunctional T state. To a slight extent, the T state is also favored by inorganic phosphate. On the other hand, adenosine 2'-monophosphate and several other 2'-phosphate nucleotides function as activators, and hence are presumed to shift the allosteric equilibrium toward the R state. The studies in this paper suggest a specific regulatory site for the transhydrogenase.
Keywords
Adenosine Monophosphate/pharmacology, Kinetics, NAD/analogs & derivatives, NADH, NADPH Oxidoreductases/*metabolism, NADP/pharmacology, Pseudomonas aeruginosa/*enzymology, Spectrometry, Fluorescence
Pubmed
Web of science
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13/08/2015 8:53
Last modification date
20/08/2019 17:23
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