Detection of malignant effusions: comparison of a telomerase assay and cytologic examination

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_F671FB2B2A59
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of malignant effusions: comparison of a telomerase assay and cytologic examination
Journal
Diagnostic Cytopathology
Author(s)
Braunschweig R., Yan P., Guilleret I., Delacretaz F., Bosman F. T., Mihaescu A., Benhattar J.
ISSN
8755-1039 (Print)
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
24
Number
3
Pages
174-180
Language
english
Notes
PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't
Abstract
Telomerase is inactive in most somatic cells, but has been found to be reactivated in a majority of cancers. Our principal goal was to test whether the presence of telomerase activity concurred with positive cytology, and was thus of potential use in detecting cancer cells in effusions. The telomeric repeat amplification protocol (TRAP) assay and cytological examination were performed in a blinded fashion on 91 unselected effusions, for which laboratory processing was done according to standard procedures. In our series, 30% (27/91) of samples were found to be malignant by cytology. Of these, 19 (70%) were also positive in the TRAP assay. Of the 8 telomerase-negative cytology-positive samples, RNA integrity was generally poor, indicating suboptimal sample conservation for molecular analysis. Negative cytology in the presence of telomerase activity was observed in 17 effusions. Of these, 11 were from patients with advanced cancer, and thus a diagnosis of malignant effusion should be suspected. The TRAP assay for telomerase activity holds promise in the analysis of effusions, but its routine use as an adjunct to cytology awaits further confirmation of its positive predictive value
Keywords
Adolescent/Adult/Aged/Aged,80 and over/Ascitic Fluid/diagnosis/enzymology/Pathology/Body Fluids/Child/Child,Preschool/Female/Humans/Male/Middle Aged/Pericardial Effusion/Pleural Effusion,Malignant/Quality Control/Telomerase/genetics/metabolism/Tumor Cells,Cultured
Pubmed
Web of science
Create date
29/01/2008 19:34
Last modification date
27/06/2020 6:20
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