Similarities and disparities between core-specific and O-side-chain-specific antilipopolysaccharide monoclonal antibodies in models of endotoxemia and bacteremia in mice
Details
Serval ID
serval:BIB_F5E9E11040C6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Similarities and disparities between core-specific and O-side-chain-specific antilipopolysaccharide monoclonal antibodies in models of endotoxemia and bacteremia in mice
Journal
Infection and Immunity
ISSN
0019-9567
Publication state
Published
Issued date
02/1997
Peer-reviewed
Oui
Volume
65
Number
2
Pages
811-4
Notes
Comparative Study
Journal Article --- Old month value: Feb
Journal Article --- Old month value: Feb
Abstract
We have previously described cross-reactive antilipopolysaccharide (anti-LPS), or anti-endotoxin, monoclonal antibodies (MAbs) which provide cross-protection in several systems of endotoxin bioactivity. The protective effects of the murine cross-reactive MAb WN1 222-5 (immunoglobulin G2a(kappa) [IgG/2a(kappa)]) and of its chimerized version, SDZ 219-800 [human IgG1(kappa)], have now been evaluated in lethality models against LPS from three different serotypes and in bacterial infection models. We confirmed the protective activity of the two MAbs in D-galactosamine-sensitized mice challenged with LPS of other E. coli serotypes (O18, O127, and O111). The protective effect correlated with the suppression of tumor necrosis factor formation. Furthermore, WN1 222-5 enhanced bacterial clearance of intravenously administered E. coli O111 bacteria, thus protecting mice from death. However, the MAbs were unable to provide protection in a peritonitis model (intraperitoneal inoculation). Our study, therefore, shows that LPS cross-reactive antibodies are capable of mediating cross-protection against LPS and bacteria but that the selected models have a clear influence on the results.
Keywords
Animals
Antibodies, Monoclonal/*chemistry
Antibody Specificity
Bacteremia/*immunology
Cross Reactions
Disease Models, Animal
Endotoxemia/*immunology
Epitopes/*immunology
Escherichia coli Infections/immunology
Female
Galactosamine/immunology
Lipopolysaccharides/*chemistry/*immunology
Mice
Pubmed
Web of science
Create date
19/01/2008 19:12
Last modification date
20/08/2019 17:22