The hepatitis C virus RNA-dependent RNA polymerase membrane insertion sequence is a transmembrane segment
Details
Serval ID
serval:BIB_F5BAE4E69D32
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The hepatitis C virus RNA-dependent RNA polymerase membrane insertion sequence is a transmembrane segment
Journal
Journal of Virology
ISSN
0022-538X (Print)
Publication state
Published
Issued date
12/2002
Volume
76
Number
24
Pages
13088-93
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp) belongs to a class of membrane proteins termed tail-anchored proteins. Here, we show that the HCV RdRp C-terminal membrane insertion sequence traverses the phospholipid bilayer as a transmembrane segment. Moreover, the HCV RdRp was found to be retained in the endoplasmic reticulum (ER) or an ER-derived modified compartment both following transient transfection and in the context of a subgenomic replicon. An absolutely conserved GVG motif was not essential for membrane insertion but possibly provides a docking site for transmembrane protein-protein interactions. These findings have important implications for the functional architecture of the HCV replication complex.
Keywords
Cell Membrane/*chemistry
Endoplasmic Reticulum/metabolism
Glycosylation
Hepacivirus/physiology
Protein Structure, Secondary
RNA Replicase/*chemistry
RNA, Viral/biosynthesis
Tumor Cells, Cultured
Viral Nonstructural Proteins/*chemistry/metabolism
Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:05
Last modification date
20/08/2019 16:22