Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_F5A355864C55
Type
Article: article from journal or magazin.
Collection
Publications
Title
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.
Journal
Nature communications
Author(s)
Gouge J., Guthertz N., Kramm K., Dergai O., Abascal-Palacios G., Satia K., Cousin P., Hernandez N., Grohmann D., Vannini A.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
25/07/2017
Peer-reviewed
Oui
Volume
8
Number
1
Pages
130
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Initiation of gene transcription by RNA polymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-binding protein), and Bdp1. TFIIIB is required for recruitment of Pol III and to promote the transition from a closed to an open Pol III pre-initiation complex, a process dependent on the activity of the Bdp1 subunit. Here, we present a crystal structure of a Brf2-TBP-Bdp1 complex bound to DNA at 2.7 Å resolution, integrated with single-molecule FRET analysis and in vitro biochemical assays. Our study provides a structural insight on how Bdp1 is assembled into TFIIIB complexes, reveals structural and functional similarities between Bdp1 and Pol II factors TFIIA and TFIIF, and unravels essential interactions with DNA and with the upstream factor SNAPc. Furthermore, our data support the idea of a concerted mechanism involving TFIIIB and RNA polymerase III subunits for the closed to open pre-initiation complex transition.Transcription initiation by RNA polymerase III requires TFIIIB, a complex formed by Brf1/Brf2, TBP and Bdp1. Here, the authors describe the crystal structure of a Brf2-TBP-Bdp1 complex bound to a DNA promoter and characterize the role of Bdp1 in TFIIIB assembly and pre-initiation complex formation.

Keywords
Amino Acid Sequence, Crystallography, X-Ray, DNA/chemistry, DNA/genetics, DNA/metabolism, Humans, Models, Molecular, Nucleic Acid Conformation, Promoter Regions, Genetic/genetics, Protein Binding, Protein Domains, RNA Polymerase III/metabolism, Sequence Homology, Amino Acid, TATA-Box Binding Protein/chemistry, TATA-Box Binding Protein/genetics, TATA-Box Binding Protein/metabolism, Transcription Factor TFIIIB/chemistry, Transcription Factor TFIIIB/genetics, Transcription Factor TFIIIB/metabolism, Transcription Initiation, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
22/08/2017 14:38
Last modification date
20/08/2019 17:22
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