Benefits of Aerosolized Phages for the Treatment of Pneumonia Due to Methicillin-Resistant Staphylococcus aureus: An Experimental Study in Rats.

Details

Serval ID
serval:BIB_F570F4032F88
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Benefits of Aerosolized Phages for the Treatment of Pneumonia Due to Methicillin-Resistant Staphylococcus aureus: An Experimental Study in Rats.
Journal
The Journal of infectious diseases
Author(s)
Prazak J., Valente L.G., Iten M., Federer L., Grandgirard D., Soto S., Resch G., Leib S.L., Jakob S.M., Haenggi M., Cameron D.R., Que Y.A.
ISSN
1537-6613 (Electronic)
ISSN-L
0022-1899
Publication state
Published
Issued date
19/04/2022
Peer-reviewed
Oui
Volume
225
Number
8
Pages
1452-1459
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The optimal method for delivering phages in the context of ventilator-associated pneumonia (VAP) is unknown. In the current study, we assessed the utility of aerosolized phages (aerophages) for experimental methicillin-resistant Staphylococcus aureus (MRSA) pneumonia.
Rats were ventilated for 4 hours before induction of pneumonia. Animals received one of the following: (1) aerophages; (2) intravenous (IV) phages; (3) a combination of IV and aerophages; (4) IV linezolid; or (5) a combination of IV linezolid and aerophages. Phages were administered at 2, 12, 24, 48, and 72 hours, and linezolid was administered at 2, 12, 24, 36, 48, 60, and 72 hours. The primary outcome was survival at 96 hours. Secondary outcomes were bacterial and phage counts in tissues and histopathological scoring of the lungs.
Aerophages and IV phages each rescued 50% of animals from severe MRSA pneumonia (P < .01 compared with placebo controls). The combination of aerophages and IV phages rescued 91% of animals, which was higher than either monotherapy (P < .05). Standard-of-care antibiotic linezolid rescued 38% of animals. However, linezolid and aerophages did not synergize in this setting (55% survival).
Aerosolized phage therapy showed potential for the treatment of MRSA pneumonia in an experimental animal model and warrants further investigation for application in humans.
Keywords
Animals, Bacteriophages, Linezolid/therapeutic use, Methicillin-Resistant Staphylococcus aureus, Pneumonia, Staphylococcal/microbiology, Pneumonia, Ventilator-Associated/drug therapy, Rats, antibiotic resistance, inhalative, nosocomial infections, phage therapy, ventilator-associated pneumonia
Pubmed
Web of science
Open Access
Yes
Create date
16/03/2021 10:14
Last modification date
27/04/2022 6:37
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