Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export.

Details

Serval ID
serval:BIB_F54EF5C21777
Type
Article: article from journal or magazin.
Collection
Publications
Title
Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export.
Journal
FEBS Letters
Author(s)
Wege S., Poirier Y.
ISSN
1873-3468 (Electronic)
ISSN-L
0014-5793
Publication state
Published
Issued date
2014
Volume
588
Number
3
Pages
482-489
Language
english
Abstract
Phosphate homeostasis in multicellular eukaryotes depends on both phosphate influx and efflux. The mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) shares homology to the Arabidopsis PHO1, a phosphate exporter expressed in roots. However, phosphate export activity of XPR1 has not yet been demonstrated in a heterologous system. Here, wedemonstrate that transient expression in tobacco leaves of XPR1-GFP leads to specific phosphate export. Like PHO1-GFP, XPR1-GFP is localized predominantly to the endomembrane system in tobacco cells. These results show that tobacco leaves are a good heterologous system to study the transport activity of members of the PHO1/XPR1 family.
Keywords
Animals, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Gene Expression Regulation, Plant, Homeostasis, Phosphates/metabolism, Plant Leaves/genetics, Plant Leaves/metabolism, Plant Roots, Plants, Genetically Modified, Receptors, G-Protein-Coupled/biosynthesis, Receptors, G-Protein-Coupled/genetics, Receptors, Virus/biosynthesis, Receptors, Virus/genetics, Tobacco/growth & development, Tobacco/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
21/08/2014 10:03
Last modification date
20/08/2019 17:22
Usage data